A scheme for sequencing large DNA molecules by identifying local nuclear-induced effects.
AUTOR(ES)
Kelson, I
RESUMO
An experimental scheme for sequencing large DNA molecules is proposed where DNA strands are replicated, with all nucleotides of a given kind marked with radioactive 32P. The marked strands are affixed to an appropriate substrate and are kept until most 32P atoms decay. The local damage caused by the decay is expected to allow the identification of the sites occupied by that particular nucleotide, using atomic scale microscopy (scanning tunneling or atomic force microscopy). Quantitative aspects and methodological considerations associated with the proposed scheme are discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=44318Documentos Relacionados
- A rhelogical separator for very large DNA molecules.
- An epistemology of nuclear weapons effects.
- Bioactive conformation of stromelysin inhibitors determined by transferred nuclear Overhauser effects.
- A model for the separation of large DNA molecules by crossed field gel electrophoresis.
- HUGE: a database for human large proteins identified by Kazusa cDNA sequencing project.
