A Reverse Genetics Approach for Recovery of Recombinant Influenza B Viruses Entirely from cDNA
AUTOR(ES)
Jackson, David
FONTE
American Society for Microbiology
RESUMO
The recovery of recombinant influenza A virus entirely from cDNA was recently described (9, 19). We adapted the technique for engineering influenza B virus and generated a mutant bearing an amino acid change E116G in the viral neuraminidase which was resistant in vitro to the neuraminidase inhibitor zanamivir. The method also facilitates rapid isolation of single-gene reassortants suitable as vaccine seeds and will aid further investigations of unique features of influenza B virus.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=136801Documentos Relacionados
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