A rapid and reliable method for the clonal isolation of Acanthamoeba from environmental samples

AUTOR(ES)

Zanella, Janice, Costa, Sergio Olavo Pinto da, Zacaria, Jucimar, Echeverrigaray, Sergio

FONTE

Brazilian Archives of Biology and Technology

DATA DE PUBLICAÇÃO

2012-02

RESUMO

Acanthamoeba are abundant in a wide range of environments, and some species are responsible for cutaneous infections, keratitis, and granulomatous amoebic encephalitis (GAE). The conventional detection and isolation of amoeba from clinical and environmental samples involves sampling and culture on non-nutrient Ágar medium. Although efficient, this system requires several transfers in order to eliminate contaminants, and is not appropriate for the isolation of individual amoeba from samples with a biodiverse community. In this study we propose an alternative method for the isolation of monocystic clones of Acanthamoeba. The propose method involves sampling, enrichment, encystment induction, and direct cysts micromanipulation and culture on Ágar plates.

Documentos Relacionados

• A rapid and reliable PCR method for detecting clonal T cell populations
• A rapid and reliable one-step method for isolating DNA fragments from agarose gels.
• A rapid and gentle method for the isolation of genomic DNA from mycobacteria.
• Immunomagnetic Capture PCR for Rapid Concentration and Detection of Hepatitis A Virus from Environmental Samples
• A Simple, Rapid, and Reliable Titrimetric Method for the Determination of Glycerol at Low Concentration