A Protein Binding Assay for Adenosine 3′:5′-Cyclic Monophosphate
AUTOR(ES)
Gilman, Alfred G.
RESUMO
A simple and sensitive assay for adenosine 3′:5′-cyclic monophosphate (cAMP) has been developed that is based on competition for protein binding of the nucleotide, presumably to a cAMP-dependent protein kinase. The nucleotide-protein complex is adsorbed on a cellulose ester filter. Assay conditions are such that a binding constant approaching 10-9 M is obtained, and the assay is thus sensitive to 0.05-0.10 pmol of cAMP.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=283204Documentos Relacionados
- Adenosine 3′:5′-cyclic monophosphate- and guanosine 3′:5′-cyclic monophosphate-dependent protein kinases: Possible homologous proteins
- Photo-Affinity Labels for Adenosine 3′:5′-Cyclic Monophosphate
- Photo-affinity labels for adenosine 3′:5′-cyclic monophosphate
- 3′:5′-Cyclic Adenosine Monophosphate Phosphodiesterase: Negative Cooperativity
- An Adenosine 3′:5′-Cyclic Monophosphate-Binding Protein That Acts on the Transcription Process