A procedure for selective DNA alkylation and detection by mass spectrometry.
AUTOR(ES)
Gut, I G
RESUMO
A method which improves the detectability of DNA by mass spectrometry is presented. By quantitatively alkylating the backbone of phosphorothioate oligonucleotides the problems of gas phase ion generation by matrix assisted laser desorption ionization can be controlled. We have developed a selective alkylating protocol for phosphorothioate oligonucleotides which is a facile way of generating non-ionic nucleic acids. A variety of alkylating agents was studied and their kinetics were monitored in a gel electrophoretic assay and by mass spectrometry.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=306863Documentos Relacionados
- DNA sequence analysis by MALDI mass spectrometry.
- Detection of aflatoxin D1 in ammoniated corn by mass spectrometry-mass spectrometry.
- Detection and identification of minor nucleotides in intact deoxyribonucleic acids by mass spectrometry.
- Protein sequencing by tandem mass spectrometry.
- Muramic acid detection in mammalian tissues by gas-liquid chromatography-mass spectrometry.