A Practical Fluorescence-Based Screening Protocol for Polyethylene Terephthalate Degrading Microorganisms
AUTOR(ES)
Chaves, Michel R. B., Lima, Maria L. S. O., Malafatti-Picca, Lusiane, de Angelis, Derlene A., Castro, Aline M. de, Valoni, Érika, Marsaioli, Anita J.
FONTE
J. Braz. Chem. Soc.
DATA DE PUBLICAÇÃO
2018-06
RESUMO
We propose a practical, low-cost and selective fluorescence-based protocol adapted to identify polyethylene terephthalate (PET) degrading microorganisms. The microbial hydrolysis of PET nanoparticles was monitored by 2-hydroxyterephthalate, a fluorophore produced in situ after radical hydroxylation of terephthalic acid (TPA), the final hydrolysis product, by the Fenton reaction. Seven fungi presenting promising PET hydrolytic potential using the proposed microscale screening assay were identified. The strains evaluated presented a substantial increase of up to 18-fold in PET nanoparticles conversion, such as obtained by the fungus Trichoderma sp. C70, after their cultivation in a PET-enriched medium. The formation of other hydrolysis products, along with TPA, was observed using matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS).
Documentos Relacionados
- Simple and Inexpensive Fluorescence-Based Technique for High-Throughput Antimalarial Drug Screening
- A fluorescence-based assay for monitoring helicase activity.
- Studies on whole cell fluorescence-based screening for epoxide hydrolases and Baeyer-Villiger monoxygenases
- Fluorescence-based DNA sequencing with hexamer primers.
- Fluorescence-based DNA sequencing with hexamer primers