A novel in vivo assay for the analysis of protein-protein interaction.

AUTOR(ES)

Maroun, M

RESUMO

The Ras Recruitment System (RRS) is a method for identification and isolation of protein-protein interaction. The method is based on translocation of cytoplasmic mammalian Ras protein to the inner leaflet of the plasma membrane through protein-protein interaction. The system is studied in a temperature-sensitive yeast strain where the yeast Ras guanyl nucleotide exchange factor is inactive at 36 degrees C. Protein-protein interaction results in cell growth at the restrictive temperature. We developed a gene reporter assay for the analysis of protein-protein interaction in mammalian cells. Ras activation in mammalian cells induces the mitogen-activated kinase cascade (MAPK), which can be monitored using Ras-dependent reporter genes. This greatly extends the usefulness of the system and provides a novel assay for protein-protein interaction in mammalian cells.

Documentos Relacionados

• The U2B'' RNP motif as a site of protein-protein interaction.
• Binding specificity and in vivo targets of the EH domain, a novel protein–protein interaction module
• A novel approach for the identification of protein–protein interaction with integral membrane proteins
• HIV-1 protease inhibits its homologous reverse transcriptase by protein-protein interaction.
• Screening for in vivo protein-protein interactions.