A Nine-Codon Deletion Mutation in the Cytomegalovirus UL97 Phosphotransferase Gene Confers Resistance to Ganciclovir

AUTOR(ES)

Chou, Sunwen

FONTE

American Society for Microbiology

RESUMO

A deletion mutation (codons 595 to 603) in the cytomegalovirus (CMV) UL97 gene was recently reported after sequence analysis of leukocyte DNA from a patient receiving ganciclovir. The corresponding viral phenotype was examined by transfer of this mutation to a laboratory CMV strain (strain Towne). The recombinant virus was resistant to ganciclovir (8.4-fold increase in the 50% inhibitory concentration), was sensitive to foscarnet, and replicated normally in cell culture.

Documentos Relacionados

• Novel mutation in the UL97 gene of a clinical cytomegalovirus strain conferring resistance to ganciclovir.
• Human cytomegalovirus UL97 kinase confers ganciclovir susceptibility to recombinant vaccinia virus.
• The Cys607→Tyr Change in the UL97 Phosphotransferase Confers Ganciclovir Resistance to Two Human Cytomegalovirus Strains Recovered from Two Immunocompromised Patients
• Mutation in the UL97 open reading frame of human cytomegalovirus strains resistant to ganciclovir.
• Mutations in human cytomegalovirus UL97 gene confer clinical resistance to ganciclovir and can be detected directly in patient plasma.