A new method to study shape recovery of red blood cells using multiple optical trapping.
AUTOR(ES)
Bronkhorst, P J
RESUMO
In this new method for studying the shape recovery of deformed red blood cells, three optical traps ("optical tweezers") induce a parachute-shaped red cell deformation, which is comparable to the deformation in small capillaries. The shape recovery is recorded, and a relaxation time is obtained for each individual red blood cell. The sensitivity of this technique for the detection of differences in relaxation times is demonstrated on subpopulations of density-separated red blood cells: "young" cells have shorter (162 ms) and "old" cells have longer (353 ms) relaxation times compared with the total population (271 ms). The relaxation time is remarkably shorter (114 ms) when the plasma surrounding the cells is replaced by a phosphate-buffered saline solution. The main advantages of this technique are the relatively short measuring and preparation time and the physiological type of deformation and shape recovery in which all relevant cell properties play a role. Therefore, especially when automated further, the technique may be a powerful tool for the study of (sub)populations of pathological red blood cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1236400Documentos Relacionados
- Wavelength dependence of cell cloning efficiency after optical trapping.
- New method for detecting changes in the surface appearance of human red blood cells
- Shape transitions of fluid vesicles and red blood cells in capillary flows
- Optical trapping and manipulation of neutral particles using lasers
- Specific isolation of 3'-terminal exons of human genes by exon trapping.