A new material to prevent urethral damage after implantation of artificial devices: an experimental study
AUTOR(ES)
Lima, Salvador Vilar Correia, Machado, Marcilio Romero, Pinto, Flávia Cristina Morone, Lira, Mariana Montenegro de Melo, Albuquerque, Amanda Vasconcelos de, Lustosa, Eugênio Soares, Silva, Jaiurte Gomes Martins da, Campos Jr., Olávio
FONTE
Int. braz j urol.
DATA DE PUBLICAÇÃO
2017-04
RESUMO
ABSTRACT Objective To validate the application of the bacterial cellulose (BC) membrane as a protecting barrier to the urethra. Materials and Methods Forty female Wistar rats (four groups of 10): Group 1 (sham), the urethra was dissected as in previous groups and nothing applied around; Group 2, received a 0.7cm strip of the BC applied around the urethra just below the bladder neck; Group 3, received a silicon strip with the same dimensions as in group 2; Group 4, had a combination of 2 and 3 groups being the silicon strip applied over the cellulosic material. Half of the animals in each group were killed at 4 and 8 months. Bladder and urethra were fixed in formalin for histological analysis. Results Inflammatory infiltrates were more intense at 4 months at lymphonodes (80% Grade 2), statistically different in the group 2 compared with groups 1 (p=0.0044) and 3 (p=0.0154). At 8 months, all samples were classified as grade 1 indicating a less intense inflammatory reaction in all groups. In group 2, at 8 months, there was a reduction in epithelial thickness (30±1μm) when com-pared to groups 1 (p=0.0001) and 3 (p<0.0001). Angiogenesis was present in groups 2 and 4 and absent in group 3. In BC implant, at 4 and 8 months, it was significant when comparing groups 4 with 1 (p=0.0159). Conclusion BC membrane was well integrated to the urethral wall promoting tissue remodeling and strengthening based on morphometric and histological results and may be a future option to prevent urethral damage.
Documentos Relacionados
- Medical devices: new regulations, new responsibilities.
- Ventricular Assist Devices: an Evolving Field
- Infections associated with indwelling devices: infections related to extravascular devices.
- Evaluation of radiographic magnification in lateral cephalograms obtained with different X-ray devices: experimental study in human dry skull
- Slow-release fluoride devices: a literature review