A new alkalophilic isolate of Bacillus as a producer of cyclodextrin glycosyltransferase using cassava flour

AUTOR(ES)

Coelho, Sheila Lorena de Araújo, Magalhães, Valter Cruz, Marbach, Phellippe Arthur Santos, Cazetta, Marcia Luciana

FONTE

Braz. J. Microbiol.

DATA DE PUBLICAÇÃO

2016-03

RESUMO

Abstract Cyclodextrin glycosyltransferase (CGTase) catalyzes the conversion of starch into non-reducing cyclic sugars, cyclodextrins, which have several industrial applications. This study aimed to establish optimal culture conditions for β-CGTase production by Bacillus sp. SM-02, isolated from soil of cassava industries waste water lake. The optimization was performed by Central Composite Design (CCD) 2, using cassava flour and corn steep liquor as substrates. The maximum production of 1087.9 U mL−1 was obtained with 25.0 g L−1 of cassava flour and 3.5 g L−1 of corn steep after 72 h by submerged fermentation. The enzyme showed optimum activity at pH 5.0 and temperature 55 °C, and maintained thermal stability at 55 °C for 3 h. The enzymatic activity was stimulated in the presence of Mg+2, Ca+2, EDTA, K+, Ba+2 and Na+ and inhibited in the presence of Hg+2, Cu+2, Fe+2 and Zn+2. The results showed that Bacillus sp. SM-02 have good potential for β-CGTase production.

Documentos Relacionados

• Isolation of alkalophilic CGTase-producing bacteria and characterization of cyclodextrin-glycosyltransferase
• Studies on alkalophilic CGTase-producing bacteria and effect of starch on cyclodextrin-glycosyltransferase activity
• Cyclodextrin formation by the thermostable alpha-amylase of Thermoanaerobacterium thermosulfurigenes EM1 and reclassification of the enzyme as a cyclodextrin glycosyltransferase.
• Isolation and characterization of new facultatively alkalophilic strains of Bacillus species.
• Produção de ciclodextrina glicosiltransferase pela bactéria alcalófila Bacillus circulans ATCC 21783 : cultivo em batelada, batelada alimentada e estado semi-sólido