A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers

AUTOR(ES)

Deprá, Maríndia, Sepel, Lenira Maria Nunes, Loreto, Élgion Lucio da Silva

FONTE

Genetics and Molecular Biology

DATA DE PUBLICAÇÃO

2004

RESUMO

We describe the transformation of white mutant Drosophila simulans with a piggyBac transposon vector and a green fluorescent marker (GFP) and show how to construct inexpensive micro-manipulation and epifluorescence equipment for use in transposon-mediated germ-line transformation. Although the number of G0 adult flies (16) obtained in relation to the number of injected eggs was very low (12.5%) it was comparable to the proportion described by other authors and can be considered as a good rate of transformation.

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