A genetic screen identifies novel non-compatible loxP sites
AUTOR(ES)
Langer, Stephen J.
FONTE
Oxford University Press
RESUMO
The ability of the Cre/lox system to make precise genomic modifications is a tremendous accomplishment. However, recombination between cis-linked heterospecific lox sites limits the use of Cre- mediated exchange of DNA to systems where genetic selection can be applied. To circumvent this problem we carried out a genetic screen designed to identify novel mutant spacer-containing lox sites displaying enhanced incompatibility with the canonical loxP site. One of the mutant sites recovered appears to be completely stable in HEK293 cells constitutively expressing Cre recombinase and supports recombinase-mediated cassette exchange (RMCE) in bacteria and mammalian cell culture. By preventing undesirable recombination, these novel lox sites could improve the efficiency of in vivo gene transfer.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=135742Documentos Relacionados
- Unidirectional Cre-mediated genetic inversion in mice using the mutant loxP pair lox66/lox71
- The role of the loxP spacer region in P1 site-specific recombination.
- Mutant loxP vectors for selectable marker recycle and conditional knock-outs
- A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast
- A highly efficient ligand-regulated Cre recombinase mouse line shows that LoxP recombination is position dependent