A first-generation packaging cell line for Epstein–Barr virus-derived vectors
AUTOR(ES)
Delecluse, Henri-Jacques
FONTE
The National Academy of Sciences
RESUMO
On the basis of the B lymphotropic Epstein–Barr virus (EBV), we have constructed a virus-free packaging cell line that allows encapsidation of plasmids into herpesvirus particles. This cell line harbors an EBV mutant whose packaging signals have been deleted. The gene vectors, which can encompass very large, contiguous pieces of foreign DNA, carry all cis-acting elements involved in amplification and encapsidation into virus-like particles as well as those essential for extrachromosomal maintenance in the recipient cell. Although this first-generation packaging cell line suffers from unwanted recombination between the helper virus genome and gene vector DNAs, this approach opens the way to delivery and stable maintenance of any transgene in human B cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=21839Documentos Relacionados
- Mapping genetic elements of Epstein-Barr virus that facilitate extrachromosomal persistence of Epstein-Barr virus-derived plasmids in human cells.
- Epstein-Barr virus-derived plasmids replicate only once per cell cycle and are not amplified after entry into cells.
- Stable alphavirus packaging cell lines for Sindbis virus- and Semliki Forest virus-derived vectors
- Efficient cDNA cloning by direct phenotypic correction of a mutant human cell line (HPRT-) using an Epstein-Barr virus-derived cDNA expression vector.
- Nondefective spleen necrosis virus-derived vectors define the upper size limit for packaging reticuloendotheliosis viruses.
