A directional, high-frequency chromosomal mobilization system for genetic mapping of Rhizobium meliloti.

AUTOR(ES)

Klein, S

RESUMO

A system for mapping of the Rhizobium meliloti chromosome that utilizes transposon Tn5-Mob, which carries the mobilization site of IncP plasmid RP4 (R. Simon, Mol. Gen. Genet. 196:413-420, 1984), was developed. Insertions of Tn5-Mob that were located at particular sites on the R. meliloti chromosome were isolated and served as origins of high-frequency chromosomal transfer when IncP tra functions were provided in trans. This approach is, in principle, applicable to any gram-negative bacterium in which Tn5 can transpose and into which IncP plasmids can conjugate.

Documentos Relacionados

• High-frequency transformation of Rhizobium meliloti.
• Genetic mapping of Rhizobium meliloti.
• High-frequency rearrangements in Rhizobium leguminosarum bv. phaseoli plasmids.
• High-frequency induction of nodulation and nitrogen fixation mutants of Rhizobium japonicum.
• Chromosomal Translocations Generated by High-Frequency Meiotic Recombination between Repeated Yeast Genes