A Differential Medium for the Enumeration of Homofermentative and Heterofermentative Lactic Acid Bacteria †

AUTOR(ES)

McDonald, L. C.

RESUMO

A medium was developed for the differential enumeration of homofermentative and heterofermentative lactic acid bacteria. Essential components of the medium included fructose (14 mM), KH2PO4 (18 mM), bromcresol green (as a pH indicator), and other nutrients to support growth. In agar medium, homofermentative colonies were blue to green, while heterofermentative colonies remained white. A total of 21 Lactobacillus, Pediococcus, Leuconostoc, and Streptococcus species were correctly classified with the medium.

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