A convenient method of aligning large DNA molecules on bare mica surfaces for atomic force microscopy.
AUTOR(ES)
Li, J
RESUMO
Large DNA molecules remain difficult to be imaged by atomic force microscopy (AFM) because of the tendency of aggregation. A method is described to align long DNA fibers in a single direction on unmodified mica to facilitate AFM studies. The clear background, minimal overstretching, high reproducibility and convenience of this aligning procedure make it useful for physical mapping of genome regions and the studies of DNA-protein complexes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=147909Documentos Relacionados
- DNA binding to mica correlates with cationic radius: assay by atomic force microscopy.
- Stretched DNA structures observed with atomic force microscopy.
- DNA condensation for gene therapy as monitored by atomic force microscopy.
- Differentiating inclusion complexes from host molecules by tapping-mode atomic force microscopy.
- DNA Deformations near Charged Surfaces: Electron and Atomic Force Microscopy Views
