1H NMR visibility of mammalian glycogen in solution.
AUTOR(ES)
Zang, L H
RESUMO
High-resolution 1H NMR spectra of rabbit liver glycogen in 2H2O were obtained at 500 MHz, and several resonances were assigned by comparison with the chemical shifts of alpha-linked diglucose molecules. The NMR relaxation times T1 and T2 of glycogen in 2H2O were determined to be 1.1 and 0.029 s, respectively. The measured natural linewidth of the carbon-1 proton (12 +/- 2 Hz) is in excellent agreement with that calculated from T2. The visibility measurements made by digesting glycogen and comparing glucose and glycogen signal intensities demonstrate that in spite of the very high molecular weight, all of the proton nuclei in glycogen contribute to the NMR spectrum. The result is not unexpected, since 100% NMR visibility was previously observed from the carbon nuclei of glycogen, due to the rapid intramolecular motions.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=53545Documentos Relacionados
- Secondary structure of the lac repressor DNA-binding domain by two-dimensional 1H nuclear magnetic resonance in solution.
- 1H NMR study of the solution structure of the self-complementary dodecanucleotide d(TGCA)3.
- Solution structure of the LexA repressor DNA binding domain determined by 1H NMR spectroscopy.
- 1H NMR STUDY OF THE HOST-GUEST CHEMISTRY IN A SUPRAMOLECULAR HELICATE FEII 2L3 SOLUTION
- Intracellular pH in human skeletal muscle by 1H NMR.
