15N NMR study of a mixture of uniformly labeled tRNAs.
AUTOR(ES)
Gonnella, N C
RESUMO
15N NMR spectra were taken of 15N-enriched tRNA extracted from bakers yeast; ammonium sulfate was used as a nitrogen source. The increase in the degree of denaturation of tRNA, which occurs with increase in temperature from 30 degrees C to 70 degrees C, resulted in no large changes in 15N chemical shifts at acidic and neutral pH but quite pronounced changes in proton-15N nuclear Overhauser effects.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=346779Documentos Relacionados
- Evidence for tautomerism in nucleic acid base pairs. 1H NMR study of 15N labeled tRNA.
- NMR of enzymatically synthesized uniformly 13C15N-labeled DNA oligonucleotides.
- Preparation and characterization of a uniformly 2 H/ 15 N-labeled RNA oligonucleotide for NMR studies.
- Study of yeast mitochondrial tRNAs by two-dimensional polyacrylamide gel electrophoresis: characterization of isoaccepting species and search for imported cytoplasmic tRNAs.
- Identity elements for N2-dimethylation of guanosine-26 in yeast tRNAs.