125I-Labeled DNA·RNA Hybrids in Cytological Preparations
AUTOR(ES)
Altenburg, L. C.
RESUMO
RNA complementary to bulk humanplacental DNA was synthesized in vitro both in the presence and absence of 3H-labeled ribonucleotides. The 3H-labeled RNA was used directly for hybridization to the DNA of human metaphase chromosomes, whereas the unlabeled complementary RNA was labeled chemically with 125I before hybridization. A comparison of autoradiographs produced by either isotope revealed no qualitative differences in the chromosomal annealing sites of the same population of RNA molecules. Since 125I-labeled nucleic acids give similar, if not identical, results as do 3H-labeled nucleic acids in in situ hybridization experiments, their use should make possible the localization of genetic elements for which tritium labeling methods are either inadequate or not possible.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=433537Documentos Relacionados
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