Argon Ion Laser
Mostrando 13-18 de 18 artigos, teses e dissertações.
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13. Automation of the Indirect Fluorescent-Antibody Test for Toxoplasmosis
The feasibility of automating the decision-making tasks of the medical technician in the determination of the results of the indirect fluorescent-antibody test for toxoplasmosis was investigated. Two approaches were studied: (i) macroscopic measurement of fluorescence from a large number of organisms (full-field illumination measurements), and (ii) microscop
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14. Application of flow cytometry to studies of pathogenic free-living amoebae.
Species of small, free-living amoebae of the genera Naegleria and Acanthamoeba can cause fatal amoebic meningoencephalitis. Previous investigations have shown that pathogenic amoebae are associated with thermally altered water. Flow cytometric techniques for identifying species of pathogenic and nonpathogenic amoebae from such water have been developed, usin
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15. Fluorescence energy transfer dye-labeled primers for DNA sequencing and analysis.
Fluorescent dye-labeled DNA primers have been developed that exploit fluorescence energy transfer (ET) to optimize the absorption and emission properties of the label. These primers carry a fluorescein derivative at the 5' end as a common donor and other fluorescein and rhodamine derivatives attached to a modified thymidine residue within the primer sequence
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16. Detection of Yersinia pestis fraction 1 antigen with a fiber optic biosensor.
A fiber optic biosensor was used to detect the fraction 1 (F1) antigen from Yersinia pestis, the etiologic agent of plague. The instrument employs an argon ion laser (514 nm) to launch light into a long-clad fiber and measures the fluorescence produced by an immunofluorescent complex formed in the evanescent wave region. This sensing area is a short section
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17. Intracellular calcium dynamics in response to action potentials in bullfrog sympathetic ganglion cells.
1. Dynamic changes in the intracellular free Ca2+ concentration ([Ca2+]i) following electrical membrane activity, were recorded from the neurone soma of the excised bullfrog sympathetic ganglion, using Fura-2 fluorescence and compared with the accompanying Ca(2+)-dependent electrical membrane responses. 2. The resting [Ca2+]i was about 100 nM, a value little
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18. Characteristics of Ca2+ release induced by Ca2+ influx in cultured bullfrog sympathetic neurones.
1. A rise in intracellular Ca2+ ([Ca2+]i) and a Ca2+ current (ICa) induced by a depolarizing pulse were simultaneously recorded by fura-2 or indo-1 fluorescence and whole-cell patch clamp techniques in cultured bullfrog sympathetic ganglion cells. 2. [Ca2+]i (calculated from the ratio of fura-2 fluorescences excited at 380 and 340 nm and recorded with a phot