Apxi
Mostrando 1-12 de 15 artigos, teses e dissertações.
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1. Diferenciação de sorotipos de Actinobacillus pleuropneumoniae pela combinação de dois PCR multiplex
A pleuropneumonia suína é uma importante doença respiratória que ocasiona grandes perdas econômicas na suinocultura. O Actinobacillus pleuropneumoniae (APP) é o agente etiológico desta enfermidade que é classificado em 15 sorotipos. Estes secretam diferentes combinações das exotoxinas ApxI, ApxII, Apx III e ApxIV, que têm sido utilizadas na difere
Cienc. Rural. Publicado em: 02/04/2013
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2. Estudo filogenético do gene apxIA de Actinobacillus pleuropneumoniae sorotipo 5 isolados no Brasil / Phylogenetic analysis of the apxIA gene of Actinobacillus pleuropneumoniae, serotype 5, isolated in Brazil
The toxins produced by Actinobacillus pleuropneumoniae (App) are recognized as major virulence factors found in this pathogen. Apx toxin I has strong hemolytic and cytotoxic activity, and this toxin has a high cytotoxic activity against pulmonary macrophages and neutrophils in swine. This exotoxin is produced by different serotypes, including the serotypes 1
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 29/04/2011
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3. Toxinas e perfil protéico de amostras de Actinobacillus suis provenientes de plantéis suínos norte-americanos
Actinobacillus suis (A.suis) surgiu como uma grande ameaça aos plantéis suínos norte-americanos. Os sinais clínicos e as lesões são particularmente variáveis e podem lembrar aquelas causadas por outros organismos, como o Actinobacillus pleuropneumoniae (App), podendo ter como causa a similaridade na produção das toxinas ApxI e ApxII. Os objetivos do
Ciência Rural. Publicado em: 17/09/2010
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4. Genotipificação de amostras sorotipificáveis e não sorotipificáveis de Actinobacillus pleuropneumoniae através de RAPD.
Actinobacillus pleuropneumoniae é o agente etiológico da pleuropneumonia suína, enfermidade amplamente distribuída no rebanho suíno mundial, responsável por prejuízos econômicos relevantes. Possui 12 sorotipos, determinados por técnicas de sorotipificação. Além disso é descrita a ocorrência de amostras não sorotipificáveis. O conhecimento do
Publicado em: 2007
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5. Structural analysis of the Actinobacillus pleuropneumoniae-RTX-toxin I (ApxI) operon.
Actinobacillus pleuropneumoniae-RTX-toxin I (ApxI), an important virulence factor, is secreted by serotypes 1, 5, 9, 10, and 11 of A. pleuropneumoniae. However, sequences homologous to the secretion genes apxIBD of the ApxI operon are present in all 12 serotypes except serotype 3. The purpose of this study was to determine and compare the structures of the A
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6. The N-Terminal Domain of RTX Toxin ApxI of Actinobacillus pleuropneumoniae Elicits Protective Immunity in Mice
We expressed three Actinobacillus pleuropneumoniae ApxI deletion derivatives to map the domain that could induce protective immunity. Antiserum to ApxI N-terminal covered by residues 40 to 380 was found to neutralize ApxI hemolytic activity but not ApxIII cytotoxicity. When used as a subunit vaccine in mice, this recombinant N-terminal fragment elicited prot
American Society for Microbiology.
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7. Knockout mutants of Actinobacillus pleuropneumoniae serotype 1 that are devoid of RTX toxins do not activate or kill porcine neutrophils.
The Actinobacillus pleuropneumoniae RTX-toxins ApxI, ApxII, and ApxIII are important virulence factors of this swine pathogen. It is hypothesized that the Apx toxins are deleterious to defense cells of the host, enabling the bacterium to infect the host. To confirm this, we studied the effect on porcine polymorphonuclear neutrophils of mutant strains of A. p
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8. Production of Apx toxins by field strains of Actinobacillus pleuropneumoniae and Actinobacillus suis.
The three Apx toxins of Actinobacillus pleuropneumoniae have potential value for use in vaccines and diagnostic tests which will be species specific instead of serotype specific, provided that the Apx toxins are species specific and all field strains produce these toxins. We examined 114 A. pleuropneumoniae field strains and found that they secreted either A
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9. Channel-forming activity and channel size of the RTX toxins ApxI, ApxII, and ApxIII of Actinobacillus pleuropneumoniae.
The determinants of the Actinobacillus pleuropneumoniae RTX toxins ApxI, ApxII, and ApxIII were expressed in an Escherichia coli strain. The toxins were concentrated from the supernatants of cell cultures. The addition of the toxins to the aqueous-phase-bathing lipid bilayer membranes resulted in an increase in the membrane conductance when membranes made of
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10. RTX toxin genotypes and phenotypes in Actinobacillus pleuropneumoniae field strains.
Actinobacillus pleuropneumoniae serotype reference strains and 204 A. pleuropneumoniae field strains representing all 12 serotypes and both biovars 1 and 2, obtained from laboratories from various countries worldwide, were analyzed for the presence of the toxin genes apxIC, apxIA, apxIB, apxID, apxIIC, apxIIA, apxIIIC, apxIIIA, apxIIIB, and apxIIID by DNA-DN
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11. Genetic map of the Actinobacillus pleuropneumoniae RTX-toxin (Apx) operons: characterization of the ApxIII operons.
Actinobacillus pleuropneumoniae RTX-toxin III (ApxIII) is implicated as an important virulence factor of A. pleuropneumoniae, the causative agent of porcine pleuropneumonia. Recently, the genes coding for ApxIII (apxIIICA) of serotype 8 were cloned and characterized. The toxin appeared to be a member of the RTX-toxin family, as are the other two secreted tox
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12. Cloning, sequencing, and regulation of a xylanase gene from the fungus Aureobasidium pullulans Y-2311-1.
Aureobasidium pullulans Y-2311-1 growing on xylan secretes four major xylanases with different masses and isoelectric points. Two of these enzymes, named APX-I and APX-II, have been purified previously. Their N-terminal amino acid sequences are identical except that APX-I has Asp and APX-II has Asn at position 7. An 83-bp DNA region was amplified by PCR and