Apparent Molecular Size
Mostrando 13-24 de 333 artigos, teses e dissertações.
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13. DNA binding of the mouse class II major histocompatibility CCAAT factor depends on two components.
A CCAAT-binding factor that recognizes a CCAAT sequence (Y box) located upstream of the major histocompatibility class II gene I-A beta has been partially purified. This CCAAT-binding factor was found to consist of two components, designated factors A and B, both of which were required for efficient binding to the DNA. Factor A had an apparent molecular size
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14. Characterization of the two size forms of the alpha 1 subunit of skeletal muscle L-type calcium channels.
The molecular properties of two size forms of the alpha 1 subunit of purified skeletal muscle calcium channels were analyzed. The minor, full-length, form, alpha 1(212), was found to have an apparent molecular mass of 214 kDa by Ferguson plot analysis, while the major, truncated, form, now designated alpha 1(190), had an apparent molecular mass of 193 kDa. A
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15. A dynorphin-like opioid in the central nervous system of an amphibian.
We have provided evidence for the existence of a biologically active opioid in toad (Bufo marinus) brain that is immunoreactive with antiserum raised against dynorphin (1-13). Compared with porcine dynorphin, this opioid is similar in apparent molecular weight on the basis of gel permeation chromatography and is more hydrophobic on the basis of high-performa
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16. Biochemical studies on the Phlebotomus fever group viruses (Bunyaviridae family).
Analyses of the virion polypeptides and genomes of several Phlebotomus fever group viruses, Karimabad, Punta Toro, Chagres, and the sandfly fever Sicilian serotype viruses, have established that they are biochemically similar to the accepted members of the Bunyaviridae family. Like snowshoe hare virus (a member of the California serogroup of the Bunyavirus g
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17. Mycoplasma fermentans-derived high-molecular-weight material induces interleukin-6 release in cultures of murine macrophages and human monocytes.
A Mycoplasma fermentans-derived high-molecular-weight material (MDHM) is described which causes differentiation of concanavalin A-stimulated CBA/J or C57BL/6 mouse thymocytes to cytolytic effector T cells (CTLs). The effect of MDHM was inhibited by addition of monoclonal anti-interleukin-6 (IL-6) antibody. It could also be abolished after removal of adherent
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18. Isolation of a low-molecular-weight serum component antigenically related to an amyloid fibril protein of unknown origin.
An amyloid fibril protein of unknown origin from a patient with systemic amyloidosis has been purified to homogeneous charge and size by gel filtration and two step isoelectric focusing. From crude antisera to the initial heterogeneous fibril protein, monospecific antibodies have been obtained by immunoabsorption with the immobilized purified amyloid protei
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19. Binding characteristics and apparent molecular size of detergent solubilized nerve growth factor receptor of sympathetic ganglia.
Nerve growth factor (NGF), a hormone-like regulator of sympathetic neuron ontogeny and metabolism affects its target cells initially by associating with specific plasma membrane receptors. We have solubilized the NGF receptor of adult rabbit superior cervical ganglia (SCG) with the nonionic detergent Triton X-100. The high-affinity equilibrium binding consta
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20. Agonist-induced increase in apparent β-adrenergic receptor size
The properties of digitonin-solubilized β-adrenergic receptors from frog erythrocyte membranes were studied by gel exclusion chromatography on AcA 34 Ultragel. β-Adrenergic receptor binding activity in these membranes can be identified by both an agonist ligand, [3H]hydroxybenzylisoproterenol, and the antagonist ligands, [3H]dihydroalprenolol and 125I-labe
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21. Arthropathic Properties Related to the Molecular Weight of Peptidoglycan-Polysaccharide Polymers of Streptococcal Cell Walls
The covalently bound polymers of peptidoglycan and group-specific polysaccharide (PG-APS) were isolated from the cell walls of group A streptococci. Arthritis was induced in rats with a single intraperitoneal injection of an aqueous suspension of PG-APS fragments derived by sonication. The joint lesions induced with this polydisperse suspension followed a bi
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22. Changes in Molecular Size Distribution of Cellulose during Attack by White Rot and Brown Rot Fungi
The kinetics of cotton cellulose depolymerization by the brown rot fungus Postia placenta and the white rot fungus Phanerochaete chrysosporium were investigated with solid-state cultures. The degree of polymerization (DP; the average number of glucosyl residues per cellulose molecule) of cellulose removed from soil-block cultures during degradation by P. pla
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23. Purification and subunit structure of the [3H]phenamil receptor associated with the renal apical Na+ channel.
Sodium crosses the apical membrane of tight epithelia through a sodium channel, which is inhibited by the diuretic amiloride and by analogs such as phenamil. Target size analysis indicated that the functional size of the [3H]phenamil binding sites associated with the epithelial Na+ channel from pig kidney is 92 +/- 10 kDa. The [3H]phenamil receptor was solub
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24. Effect of RNase III on the size of bacteriophage T7 lysozyme mRNA.
The size of lysozyme mRNA from T7-infected E. coli RNase III+ and RNase III- strains was analyzed by sucrose gradient sedimentation, dimethylsulfoxide (Me2SO) sucorse gradient sedimentation, and preparative gel electrophoresis. Each technique revealed a similar size distribution of multiple lysozyme mRNA's. Analysis by preparative gel electrophoresis of RNA