Apium Graveolens
Mostrando 13-24 de 28 artigos, teses e dissertações.
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13. Estimation of Polymer Rigidity in Cell Walls of Growing and Nongrowing Celery Collenchyma by Solid-State Nuclear Magnetic Resonance in Vivo.
When the growth of a plant cell ceases, its walls become more rigid and lose the capacity to extend. Nuclear magnetic resonance relaxation methods were used to determine the molecular mobility of cell wall polymers in growing and nongrowing live celery (Apium graveolens L.) collenchyma. To our knowledge, this is the first time this approach has been used in
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14. Subcellular localization of celery mannitol dehydrogenase. A cytosolic metabolic enzyme in nuclei.
Mannitol dehydrogenase (MTD) is the first enzyme in mannitol catabolism in celery (Apium graveolens L. var dulce [Mill] Pers. cv Florida 638). Mannitol is an important photoassimilate, as well as providing plants with resistance to salt and osmotic stress. Previous work has shown that expression of the celery Mtd gene is regulated by many factors, such as he
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15. Tissue Printing as a Tool for Observing Immunological and Protein Profiles in Young and Mature Celery Petioles.
Tissue printing onto membranes such as nitrocellulose is a technique employed to study the localization of proteins, nucleic acids, and soluble metabolites from freshly cut tissue slices. We probed tissue prints of young and mature celery (Apium graveolens) petioles with antibodies raised against two proteins, spinach ribulose-1,5-bisphosphate carboxylase an
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16. Immunolocalization of mannitol dehydrogenase in celery plants and cells.
Immunolocalization of mannitol dehydrogenase (MTD) in celery (Apium graveolens L.) suspension cells and plants showed that MTD is a cytoplasmic enzyme. MTD was found in the meristems of celery root apices, in young expanding leaves, in the vascular cambium, and in the phloem, including sieve-element/companion cell complexes, parenchyma, and in the exuding ph
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17. Sugar Repression of Mannitol Dehydrogenase Activity in Celery Cells.
We present evidence that the activity of the mannitol-catabolizing enzyme mannitol dehydrogenase (MTD) is repressed by sugars in cultured celery (Apium graveolens L.) cells. Furthermore, this sugar repression appears to be mediated by hexokinases (HKs) in a manner comparable to the reported sugar repression of photosynthetic genes. Glucose (Glc)-grown cell c
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18. Facilitated Transport of Glucose in Isolated Phloem Segments of Celery 1
In isolated phloem segments of celery (Apium graveolens L.), a tissue highly specific for sucrose and mannitol uptake, glucose uptake occurs at very low rates and exhibits biphasic kinetics. Nonpenetrating inhibitors such as parachloromercuribenzene sulfonic acid did not inhibit glucose uptake. However, uptake was greatly inhibited by penetrating inhibitors
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19. Diversity of the Superfamily of Phloem Lectins (Phloem Protein 2) in Angiosperms1
Phloem protein 2 (PP2) is one of the most abundant and enigmatic proteins in the phloem sap. Although thought to be associated with structural P-protein, PP2 is translocated in the assimilate stream where its lectin activity or RNA-binding properties can exert effects over long distances. Analyzing the diversity of these proteins in vascular plants led to th
American Society of Plant Biologists.
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20. Molecular cloning of mannose-6-phosphate reductase and its developmental expression in celery.
Compared with other primary photosynthetic products (e.g. sucrose and starch), little is known about sugar alcohol metabolism, its regulation, and the manner in which it is integrated with other pathways. Mannose-6-phosphate reductase (M6PR) is a key enzyme that is involved in mannitol biosynthesis in celery (Apium graveolens L.). The M6PR gene was cloned fr
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21. Purification of NAD-dependent mannitol dehydrogenase from celery suspension cultures.
Mannitol dehydrogenase, a mannitol:mannose 1-oxidoreductase, constitutes the first enzymatic step in the catabolism of mannitol in nonphotosynthetic tissues of celery (Apium graveolens L.). Endogenous regulation on the enzyme activity in response to environmental cues is critical in modulating tissue concentration of mannitol, which, importantly, contribute
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22. Mannitol Metabolism in Celery Stressed by Excess Macronutrients.
The effect of excess macronutrients in the root environment on mannitol and sucrose metabolism was investigated in celery (Apium graveolens L. var dulce [Mill.] Pers.). Plant growth was inhibited progressively as macronutrient concentration in the media, as measured by electrical conductivity (E.C.), increased from 1.0 to 11.9 decisiemens m-1. Plants grown f
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23. The Calcium-Binding Activity of a Vacuole-Associated, Dehydrin-Like Protein Is Regulated by Phosphorylation1
A vacuole membrane-associated calcium-binding protein with an apparent mass of 45 kD was purified from celery (Apium graveolens). This protein, VCaB45, is enriched in highly vacuolate tissues and is located within the lumen of vacuoles. Antigenically related proteins are present in many dicotyledonous plants. VCaB45 contains significant amino acid identity w
American Society of Plant Physiologists.
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24. Interaction of Cell Turgor and Hormones on Sucrose Uptake in Isolated Phloem of Celery 1
Phloem tissue isolated from celery (Apium graveolens L.) was used to investigate the regulation of sucrose uptake by turgor (manipulated by 50-400 milliosomolal solutions of polyethylene glycol) and hormones indoleacetic acid (IAA) and gibberillic acid (GA3). Sucrose uptake was enhanced under low cellular turgor (increase in the Vmax). Furthermore, enhanceme