Antibiotics Microbial Assay
Mostrando 1-9 de 9 artigos, teses e dissertações.
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1. Synergistic antibacterial action of β-sitosterol-D-glucopyranoside isolated from Desmostachya bipinnata leaves with antibiotics against common human pathogens
Desmostachya bipinnata (L.) Stapf, Poaceae, or Kusha in Sanskrit, is a sacred grass used extensively in Indian Vedic practices. It is well known for its medicinal value and is used in traditional Indian medicine to treat microbial infection in combination with other herbs. An effort has been made to isolate and characterize the bioactive compounds from the h
Rev. bras. farmacogn.. Publicado em: 2014-01
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2. Doseamento microbiológico de apramicina - desenvolvimento e validação de método empregando leitura cinética em microplacas / Microbial assay of apramycin - delevopment and validation of assay using kinetic-reading microplate system
O princípio do ensaio turbidimétrico é simples: a solução-teste é adicionada à suspensão do microrganismo-teste em meio de cultura, a mistura é incubada em condições adequadas e o crescimento microbiano é medido através da leitura fotométrica. O emprego de método de microplacas com leitura cínética para a dosagem de antibióticos é de inter
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 16/10/2009
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3. The Calgary Biofilm Device: New Technology for Rapid Determination of Antibiotic Susceptibilities of Bacterial Biofilms
Determination of the MIC, based on the activities of antibiotics against planktonic bacteria, is the standard assay for antibiotic susceptibility testing. Adherent bacterial populations (biofilms) present with an innate lack of antibiotic susceptibility not seen in the same bacteria grown as planktonic populations. The Calgary Biofilm Device (CBD) is describ
American Society for Microbiology.
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4. Inhibition of Acetylating Activity in an Enzymatic Assay for Aminoglycoside Antibiotics
The radioenzymatic technique using aminoglycoside-6′-acetyltransferase is suitable for determining serum concentrations of aminoglycoside antibiotics that have a 6′-amino group. Standard curves constructed for each drug in normal human serum are generally satisfactory, but we encountered a pool of human serum (designated IN pool) that inhibited the acety
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5. A Simple Methodological Approach for Counting and Identifying Culturable Viruses Adsorbed to Cellulose Nitrate Membrane Filters
We identified conditions under which Buffalo green monkey cells grew on the surfaces of cellulose nitrate membrane filters in such a way that they covered the entire surface of each filter and penetrated through the pores. When such conditions were used, poliovirus that had previously been adsorbed on the membranes infected the cells and replicated. A plaque
American Society for Microbiology.
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6. Use of the Microbial Growth Curve in Postantibiotic Effect Studies of Legionella pneumophila
Using the standard Craig and Gudmundsson method (W. A. Craig and S. Gudmundsson, p. 296-329, in V. Lorian, ed., Antibiotics in Laboratory Medicine, 1996) as a guideline for determination of postantibiotic effects (PAE), we studied a large series of growth curves for two strains of Legionella pneumophila. We found that the intensity of the PAE was best determ
American Society for Microbiology.
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7. Distribution of Biosurfactant-Producing Bacteria in Undisturbed and Contaminated Arid Southwestern Soils
Biosurfactants are a unique class of compounds that have been shown to have a variety of potential applications in the remediation of organic- and metal-contaminated sites, in the enhanced transport of bacteria, in enhanced oil recovery, as cosmetic additives, and in biological control. However, little is known about the distribution of biosurfactant-produci
American Society for Microbiology.
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8. The role of human β-defensin-2 in bone
Osteomyelitis often causes functional impairment due to tissue destruction. This report demonstrates a novel previously unappreciated role of osteoblasts. Samples of osteomyelitic bone and bacterially challenged osteoblasts produce increased amounts of antimicrobial peptides in order to combat bacterial bone infection. An osteomyelitis mouse model confirmed
Blackwell Science Inc.
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9. Enhancement of Neonatal Innate Defense: Effects of Adding an N-Terminal Recombinant Fragment of Bactericidal/Permeability-Increasing Protein on Growth and Tumor Necrosis Factor-Inducing Activity of Gram-Negative Bacteria Tested in Neonatal Cord Blood Ex Vivo
Innate defense against microbial infection requires the action of neutrophils, which have cytoplasmic granules replete with antibiotic proteins and peptides. Bactericidal/permeability-increasing protein (BPI) is found in the primary granules of adult neutrophils, has a high affinity for lipopolysaccharides (or “endotoxins”), and exerts selective cytotoxi
American Society for Microbiology.