Animal Enteritis
Mostrando 13-24 de 32 artigos, teses e dissertações.
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13. Atypical cpb2 Genes, Encoding Beta2-Toxin in Clostridium perfringens Isolates of Nonporcine Origin
Beta2-toxin, encoded by cpb2, is implicated in the pathogenesis of Clostridium perfringens enteritis. However, cpb2 genes from nonporcine C. perfringens isolates were not always expressed, at least in vitro. Nucleotide sequencing identified atypical cpb2 genes with 70.2 to 70.7% DNA identity to previously identified (consensus) cpb2. Atypical beta2-toxin dis
American Society for Microbiology.
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14. Persistent Endemicity of Salmonella bongori 48:z35:− in Southern Italy: Molecular Characterization of Human, Animal, and Environmental Isolates
From 1984 to 1999, we collected 31 isolates of the rare serovar Salmonella bongori 48:z35:− in southern Italy. Twenty-four of the isolates were from cases of acute enteritis in humans. Pulsed-field gel electrophoresis analysis showed that all but one of our isolates were at least 80% similar. Our findings suggest that genetically related S. bongori 48:z35:
American Society for Microbiology.
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15. Astrovirus Induces Diarrhea in the Absence of Inflammation and Cell Death
Astroviruses are a leading cause of infantile viral gastroenteritis worldwide. Very little is known about the mechanisms of astrovirus-induced diarrhea. One reason for this is the lack of a small-animal model. Recently, we isolated a novel strain of astrovirus (TAstV-2) from turkeys with the emerging infectious disease poult enteritis mortality syndrome. In
American Society for Microbiology.
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16. Campylobacter cinaedi is normal intestinal flora in hamsters.
During the course of studies to reproduce proliferative enteritis in hamsters, Campylobacter cinaedi was recovered from the feces of the majority of healthy hamsters obtained from two commercial sources. The organisms were cultured by using filtration, a nonselective medium, and a microaerophilic atmosphere containing hydrogen. Isolation was hindered by the
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17. Effects of Tylosin on Bacterial Mucolysis, Clostridium perfringens Colonization, and Intestinal Barrier Function in a Chick Model of Necrotic Enteritis
Necrotic enteritis (NE) is a worldwide poultry disease caused by the alpha toxin-producing bacterium Clostridium perfringens. Disease risk factors include concurrent coccidial infection and the dietary use of cereal grains high in nonstarch polysaccharides (NSP), such as wheat, barley, rye, and oats. Outbreaks of NE can be prevented or treated by the use of
American Society for Microbiology.
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18. Experimental Campylobacter diarrhea in chickens.
An animal model for Campylobacter fetus subsp. jejuni enteritis was developed in 3-day-old chickens. Diarrhea was induced in 88% (22 of 25) of chickens inoculated with 9 X 10(7) bacteria given orally. The mean incubation time was 45 h (range, 24 to 72 h). Considerable weight loss was observed in the experimental group compared with the control group. Ninety
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19. Neurokinin-1 (NK-1) receptor is required in Clostridium difficile- induced enteritis.
Toxin A, a 308,000-Mr enterotoxin from Clostridium difficile, mediates antibiotic-associated diarrhea and colitis in humans. Injection of toxin A into animal intestine triggers an acute inflammatory response characterized by activation of sensory neurons and immune cells of the intestinal lamina propria, including mast cells and macrophages, and migration of
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20. Use of a Monoclonal Antibody against an Escherichia coli O26 Surface Protein for Detection of Enteropathogenic and Enterohemorrhagic Strains
A monoclonal antibody (MAb) was obtained from a mouse immunized with solubilized outer membrane proteins extracted from a bovine enterohemorrhagic strain of Escherichia coli (EHEC), O26. The MAb produced a strong immunoblot reaction at approximately 21 kDa for an O26 strain containing the intimin gene (eae) and verocytotoxin (VT), but not with an O26 ea
American Society for Microbiology.
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21. Evidence for numerous omp1 alleles of porcine Chlamydia trachomatis and novel chlamydial species obtained by PCR.
A nested PCR for genus-specific amplification of the Chlamydia omp1 locus was established. This PCR detected single template molecules in 200-microl specimen aliquots. Amplified chlamydial omp1 alleles were typed by heminested species PCRs and allele PCRs. We applied this method to 407 specimens from several host animals with various clinical conditions, and
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22. Evidence for two types of cytotoxic necrotizing factor in human and animal clinical isolates of Escherichia coli.
We have characterized the in vitro and in vivo toxic properties of cell sonic extracts from 22 animal and human clinical isolates of Escherichia coli that caused both necrosis in the rabbit skin and multinucleation in tissue cultures, two toxic properties previously reported as being specific for E. coli cytotoxic necrotizing factor (CNF). Two distinct toxic
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23. Atypical Yersinia enterocolitica: clinical and epidemiological parameters.
Infections due to biochemically typical Yersinia enterocolitica usually present as gastroenteritis, mesenteric lymphadenitis, terminal ileitis, and septicemia often with visceral abscesses. In these instances, the isolates have been biochemically typical and of well-established serotypes, namely 0:3 or 0:9 and, in the United States, 0:5 or 0:8. The recovery,
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24. Species-specific cloned DNA probes for the identification of Campylobacter hyointestinalis.
Conventional microbiological methods for isolating and identifying Campylobacter species are laborious, tedious, and subjective. Because of the increasing importance of Campylobacter species in human and animal diseases and the recent emergence of many new species and atypical strains, we are developing chromosomal DNA probes for rapid and simple identificat