Aleurone
Mostrando 1-12 de 233 artigos, teses e dissertações.
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1. Analysis, classification, annotation and expression pattern of transcription factors in maize (Zea mays L.) endosperm / Analise, classificação, anotação e perfil de expressão de fatores de transcrição no endosperma de milho (Zea mays L.)
O seqüenciamento de ESTs (etiquetas de seqüências expressas) e a sua organização em bancos de dados constituem poderosas ferramentas para identificar genes de interesse expressos em determinados tecidos e/ou tipos celulares. Neste trabalho criou-se um banco de seqüências expressas chamado MAIZESTdb, que contém ESTs de diversos tecidos de milho, poré
Publicado em: 2006
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2. Endo-beta-mananase de endosperma de Sesbania virgata (Cv.) Pers : purificação, caracterização e importancia na germinação e desenvolvimento da plantula
Many plant seeds have an endosperm, a tissue specialised either as storage or mechanical constraint to embryo growth. In many legume species, endosperms contain galactomannan. This polyssacharide is composed of a main chain of 1,4-β-mannan branched with variable amounts of 1,6-ά-linked galactosyl residues. Three enzymes produced by an aleurone laye
Publicado em: 2003
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3. Gibberellic Acid Enhancement of DNA Turnover in Barley Aleurone Cells 1
When imbibed, deembryonated halfseeds from barley (Hordeum vulgare L., var. Himalaya) are incubated in buffer, the DNA content of the aleurone layer increases 25 to 40% over a 24-hour period. In contrast, the DNA of isolated aleurone layers declines by 20% over the same time period. Gibberellic acid (GA) causes a reduction in DNA levels in both halfseed aleu
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4. On the Uptake, Metabolism and Retention of [3H] Gibberellin A1 by Barley Aleurone Layers at Low Temperatures 1
Barley (c.v. Himalaya) aleurone layers were incubated in [3H]gibberellin A1 (GA1) at low temperatures. At 3 and 4 C, 3H-activity was steadily accumulated in aleurone layers, and this accumulation was correlated with significant [3H]GA1 metabolism. At 1 and 1.5 C, metabolism could not be detected, and at these temperatures aleurone layers equilibrated with th
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5. Gibberellic Acid Induces Vacuolar Acidification in Barley Aleurone.
The roles of gibberellic acid (GA3) and abscisic acid (ABA) in the regulation of vacuolar pH (pHv) in aleurone cells of barley were investigated using the pH-sensitive fluorescent dye 2[prime],7[prime]-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF). BCECF accumulated in vacuoles of aleurone cells, but sequestration of the dye did not affect its sensitiv
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6. Aleurone Cell Identity Is Suppressed following Connation in Maize Kernels1
Expression of the cytokinin-synthesizing isopentenyl transferase enzyme under the control of the Arabidopsis (Arabidopsis thaliana) SAG12 senescence-inducible promoter reverses the normal abortion of the lower floret from a maize (Zea mays) spikelet. Following pollination, the upper and lower floret pistils fuse, producing a connated kernel with two genetica
American Society of Plant Biologists.
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7. The regulation of gene expression in transformed maize aleurone and endosperm protoplasts. Analysis of promoter activity, intron enhancement, and mRNA untranslated regions on expression.
Gene expression in the aleurone and endosperm is highly regulated during both seed development and germination. Studies of alpha-amylase expression in the aleurone of barley (Hordeum vulgare) have generated the current paradigm for hormonal control of gene expression in germinating cereal grain. Gene expression studies in both the aleurone and endosperm tiss
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8. Endoplasmic Reticulum Formation during Germination of Wheat Seeds 1: A QUANTITATIVE ELECTRON MICROSCOPE STUDY
This study demonstrates germination-induced ultrastructural changes in wheat (Triticum aestivum L. cv Arthur) aleurone cells. Seeds imbided for 4 hours in water contained endoplasmic reticulum (ER) or ER-like membranes as vesicles or as short segments of membrane associated with the spherosomes on the periphery of aleurone grains. Aleurone cells incubated be
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9. Responses of Enzymically Isolated Aleurone Cells of Oat to Gibberellin A31
Oat (Avena sativa L.) aleurone layer cells (spheroplasts) were isolated by maceration of the aleurone layer with a mixture of commercially available cellulase and pectinase. About 20% of the cells present in intact layers were released as spheroplasts and 79 ± 9% of the spheroplast population was viable as judged by methylene blue staining. The spheroplasts
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10. Kinetics of the Acidification Capacity of Aleurone Layer and Its Effect upon Solubilization of Reserve Substances from Starchy Endosperm of Wheat
The capacity of the isolated barley aleurone layer for endosperm acidification has been demonstrated (J Mikola, M Virtanen 1980 Plant Physiol 66: S-142). The kinetics of this acidification by isolated wheat aleurone layer and its effect on starchy endosperm solubilization are reported.
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11. sal1 determines the number of aleurone cell layers in maize endosperm and encodes a class E vacuolar sorting protein
A microscopy-based screen of a large collection of maize Mutator (Mu) transposon lines identified the supernumerary aleurone layers 1-1 (sal1-1) mutant line carrying up to seven layers of aleurone cells in defective kernel endosperm compared with only a single layer in wild-type grains. Normal, well filled endosperm that is homozygous for the sal1-1 mut
National Academy of Sciences.
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12. Enzymes That Scavenge Reactive Oxygen Species Are Down-Regulated Prior to Gibberellic Acid-Induced Programmed Cell Death in Barley Aleurone1
Gibberellins (GAs) initiate a series of events that culminate in programmed cell death, whereas abscisic acid (ABA) prevents this process. Reactive oxygen species (ROS) are key elements in aleurone programmed cell death. Incubation of barley (Hordeum vulgare) aleurone layers in H2O2 causes rapid death of all cells in GA- but not ABA-treated layers. Sensitivi
American Society of Plant Physiologists.