Adeno Associated Virus
Mostrando 1-12 de 488 artigos, teses e dissertações.
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1. Nanopartículas poliméricas de PLGA no tratamento de carcinoma hepatocelular e câncer colo-retal / PLGA polymeric nanoparticles in the treatment of hepatocellular carcinoma and colorectal cancer
Neste trabalho preparamos e utilizamos nanopartículas de PLGA-DOTAP encapsulando o peptídeo P17, que bloqueia a ação da citocina imunossupressora TGF-b, no tratamento de camundongos BALB/c fêmea, inoculados via subcutânea com células de hepatocarcinoma (CHC) murino BNL ou de câncer colo-retal (CCR) murino CT26. No modelo de CHC, nanopartículas foram
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 09/02/2011
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2. Adeno-associated virus for cystic fibrosis gene therapy
Gene therapy is an alternative treatment for genetic lung disease, especially monogenic disorders such as cystic fibrosis. Cystic fibrosis is a severe autosomal recessive disease affecting one in 2500 live births in the white population, caused by mutation of the cystic fibrosis transmembrane conductance regulator (CFTR). The disease is classically character
Brazilian Journal of Medical and Biological Research. Publicado em: 2011-11
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3. A role for adeno-associated viral vectors in gene therapy
Gene therapy constitutes a therapeutic intervention based on modification of the genetic material of living cells, by correcting genetic defects or overexpressing therapeutic proteins. The success of gene therapy protocols depends on the availability of therapeutically suitable genes, appropriate gene delivery systems and proof of safety and efficacy. Recent
Publicado em: 2010
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4. The state of the art of adeno-associated virus-based vectors in gene therapy
The adeno-associated virus (AAV) has rapidly gained popularity in gene therapy since the establishment of the first AAV2 infectious clone, in 1982, due to some of their distinguishing characteristics such as lack of pathogenicity, wide range of infectivity, and ability to establish longterm transgene expression. Notably over the past decade, this virus has a
Publicado em: 2010
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5. Construção e caracterização de um vírus Adeno-associado com expressão direcionada para células em divisão / Construction and characterization of adeno-associated virus with limited expression for proliferating cells
A utilização do vírus adeno-associado recombinante (AAVr) como vetor de transferência gênica em células tumorais está crescendo. Neste trabalho, o promotor gênico de E2F-1, um promotor ativo durante a divisão celular, foi inserido no AAVr e utilizado para dirigir a expressão do HSV-tk ou luciferase e, simultaneamente, eGFP afim de direcionar a expr
Publicado em: 2010
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6. A role for adeno-associated viral vectors in gene therapy
Gene therapy constitutes a therapeutic intervention based on modification of the genetic material of living cells, by correcting genetic defects or overexpressing therapeutic proteins. The success of gene therapy protocols depends on the availability of therapeutically suitable genes, appropriate gene delivery systems and proof of safety and efficacy. Recent
Genetics and Molecular Biology. Publicado em: 2008
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7. Abortamento humano : detecção molecular de AAV e de HPV em decídua e vilosidade coriônica
Pregnancy failure is a common event often of unknown cause. Some viruses are suggested as cause of abortion and, among them, adeno-associated virus (AAV), that has never been implicated as a cause of disease. AAV (AAV1-11 types), belonging to Parvoviridae family, requires helper virus function for replication, as from human papillomavirus (HPV), that belongs
Publicado em: 2007
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8. Somatic gene therapy for hypertension
Gene therapy for hypertension is needed for the next generation of antihypertensive drugs. Current drugs, although effective, have poor compliance, are expensive and short-lasting (hours or one day). Gene therapy offers a way to produce long-lasting antihypertensive effects (weeks, months or years). We are currently using two strategies: a) antisense oligode
Brazilian Journal of Medical and Biological Research. Publicado em: 2000-06
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9. Adeno-associated virus vectors preferentially transduce cells in S phase.
Vectors based on adeno-associated virus can stably transfer genes by chromosomal integration in recipient cells. In this study we have infected stationary and dividing primary human fibroblast cultures with adeno-associated virus vectors encoding alkaline phosphatase and neomycin phosphotransferase. We find that the transduction frequency of S phase cells is
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10. Mapping of the 5' termini of two adeno-associated virus 2 RNAs in the left half of the genome.
The left 45% of the adeno-associated virus 2 genome was sequenced. The 5' termini of two adeno-associated virus-specific RNAs were mapped at the nucleotide level within this region of the genome (nucleotides 286 to 288 and 871 to 874). Both of these 5' termini map 31 +/- 2 nucleotides downstream from the start of "TATA' boxes.
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11. Site-Specific Integration of Adeno-Associated Virus into an Episome with the Target Locus via a Deletion-Substitution Mechanism
Five site-specific adeno-associated virus integrants generated in a model system with an Epstein-Barr virus- based shuttle vector have been characterized. The results suggest a deletion-substitution mechanism of recombination.
American Society for Microbiology.
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12. Separation of Two Types of Adeno-Associated Virus Particles Containing Complementary Polynucleotide Chains
An adeno-associated virus containing bromodeoxyuridine-substituted deoxyribonucleic acid has been fractionated by equilibrium centrifugation in CsCl into two classes of virions which contain complementary polynucleotide chains.