Acidic Phospholipases A2
Mostrando 1-9 de 9 artigos, teses e dissertações.
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1. Cytotoxic and inflammatory potential of a phospholipase A2 from Bothrops jararaca snake venom
Abstract Background: Snake venom phospholipases A2 (PLA2s) have been reported to induce myotoxic, neurotoxic, hemolytic, edematogenic, cytotoxic and proinflammatory effects. This work aimed at the isolation and functional characterization of a PLA2 isolated from Bothrops jararaca venom, named BJ-PLA2-I. Methods and Results: For its purification, three cons
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 17/12/2018
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2. Purification and enzymatic characterization of a novel metalloprotease from Lachesis muta rhombeata snake venom
Abstract Background: Lachesis muta rhombeata (Lmr) is the largest venomous snake in Latin America and its venom contains mainly enzymatic components, such as serine and metalloproteases, L-amino acid oxidase and phospholipases A2. Metalloproteases comprise a large group of zinc-dependent proteases that cleave basement membrane components such as fibronectin
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 17/12/2018
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3. Caracterização funcional e estrutural de uma nova fosfolipase A2 ácida de Bothrops moojeni / Functional and structural characterization of a new acidic phospholipase A2 from Bothrops moojeni
The phospholipase A2 (PLA2s) are enzymes that induce various pharmacological effects and usually correspond to a higher percentage of the protein content of snake venoms. Thus, isolation, biochemical, functional and structural characterization of PLA2s may generate important information for a better understanding of the pharmacological effects and toxicity i
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/01/2012
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4. Biochemical and toxinological characterization of Naja sumatrana (Equatorial spitting cobra) venom
The lethal and enzymatic activities of venom from Naja sumatrana (Equatorial spitting cobra) were determined and compared to venoms from three other Southeast Asian cobras (Naja sputatrix, Naja siamensis and Naja kaouthia). All four venoms exhibited the common characteristic enzymatic activities of Asiatic cobra venoms: low protease, phosphodiesterase, alkal
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2011
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5. Functional and Structural Characterization of an Acidic Toxic Phospholipase A2 from Bothrops moojeni Snake Venom. / Caracterização funcional e estrutural de uma fosfolipase A2 ácida tóxica isolada da peçonha de Bothrops moojeni
As fosfolipases A2 (PLA2s, E.C. 3.1.1.4) pertencem a uma superfamília de enzimas que realizam a clivagem de fosfolipídios da membrana celular em ácidos graxos e lisofosfolipídios, numa reação dependente de cálcio. As PLA2s apresentam um importante papel em várias funções celulares, incluindo manutenção dos fosfolipídios celulares, geração de p
Publicado em: 2009
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6. Purificação e caracterização de uma nova fosfolipase A2 ácida da peçonha de Bothrops pauloensis
Phospholipases A2 (PLA2) is small enzymes that present molecular mass around 15kDa and are found in a variety of biological and cellular fluids as fluid sinovial, macrophages, platelet, pancreatic tissue, among others. The present work describes the purification and chemical, biological and enzymatic characterization of an acidic phospholipase A2 of Bothrops
Publicado em: 2006
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7. PurificaÃÃo e caracterizaÃÃo de uma nova fosfolipase A2 Ãcida da peÃonha de Bothrops pauloensis
Phospholipases A2 (PLA2) is small enzymes that present molecular mass around 15kDa and are found in a variety of biological and cellular fluids as fluid sinovial, macrophages, platelet, pancreatic tissue, among others. The present work describes the purification and chemical, biological and enzymatic characterization of an acidic phospholipase A2 of Bothrops
Publicado em: 2006
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8. Regulation of phospholipase A2 in human leukemia cell lines: its implication for intracellular signaling.
Permeabilized human leukemia HL-60 and U-937 cells suspended in an acidic or alkaline medium release various unsaturated fatty acids, most abundantly oleic and arachidonic acids. Concomitant production of lysophospholipids suggests that phospholipases A2 play a major role in this fatty acid release reaction. The fatty acid release at acidic conditions depend
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9. Action of Phospholipase A2 and Phospholipase C on Bacillus subtilis Protoplasts
Protoplasts prepared from Bacillus subtilis by lysozyme digestion lysed in the presence of pure pancreatic phospholipase A2. The phospholipids cardiolipin, phosphatidylethanolamine, phosphatidylglycerol and lysylphosphatidylglycerol, which are present in the membrane, are degraded by phospholipase A2 only after removal of the cell wall, giving free fatty aci