A Amylase Inhibitors
Mostrando 13-24 de 33 artigos, teses e dissertações.
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13. Variantes de inibidores de [alfa]-amilases selecionados por Phage Display com atividade para [alfa]-amilases do bicudo do algodoeiro (Anthonomus grandis, coleptera: curculionidae)
The cotton boll weevil Anthonomus grandis is an important insect-pest, that causes severe damage in cotton floral fruits and buds. The A. gradis larvae development control is a difficult job because it occurs inside the cotton floral structures. Starch granules from cotton structure are digested by a-amylases secreted to the intestinal lumen. However, a-amyl
Publicado em: 2007
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14. General Biochemical Characterization of Thermostable Extracellular β-Amylase from Clostridium thermosulfurogenes
Clostridium thermosulfurogenes, an anaerobic bacterium which ferments starch into ethanol at 62°C, produced an active extracellular amylase and contained intracellular glucoamylase but not pullulanase activity. The extracellular amylase was purified 2.4-fold, and its general physicochemical and catalytic properties were examined. The extracellular amylase w
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15. A lectin gene encodes the alpha-amylase inhibitor of the common bean.
An alpha-amylase inhibitor that inhibits insect and mammalian alpha-amylases but not plant alpha-amylases, is present in seeds of the common bean (Phaseolus vulgaris). We have purified the alpha-amylase inhibitor by using a selective heat treatment in acidic medium and affinity chromatography with porcine pancreas alpha-amylase coupled to agarose. Under sodi
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16. Bean α-amylase inhibitor 1 in transgenic peas (Pisum sativum) provides complete protection from pea weevil (Bruchus pisorum) under field conditions
Two α-amylase inhibitors, called αAI-1 and αAI-2, that share 78% amino acid sequence identity and have a differential specificity toward mammalian and insect α-amylases are present in different accessions of the common bean (Phaseolus vulgaris). Using greenhouse-grown transgenic peas (Pisum sativum), we have shown previously that expression of αAI-1 in
The National Academy of Sciences.
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17. Inhibitors of Protein Phosphatases 1 and 2A Block the Sugar-Inducible Gene Expression in Plants.
Genes coding for two major proteins of the tuberous root of sweet potato (Ipomoea batatas), namely, sporamin and [beta]-amylase, are inducible in leaves and petioles when they are supplied with high concentrations of sucrose or other metabolizable sugars, such as glucose and fructose, and the accumulation of a large amount of starch accompanies this inductio
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18. Release and Activity of Bound β-Amylase in a Germinating Barley Grain 1
In resting grains of Triumph barley (Hordeum vulgare L. cv Triumph) about 40% of the β-amylase could be extracted with a saline solution, the remaining 60% being in a bound form. During seedling growth (20°C), the bound form was released mainly between days 1 and 3. When a preparation containing bound β-amylase was incubated with an extract made of endosp
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19. Embryoless Wheat Grain: A Natural System for the Study of Gibberellin-induced Enzyme Formation 1
Yorkstar wheat, grown in New York State, has a high percentage (10-11) of grains without embryos. The embryoless grains have viable aleurone layers and show no sign of injury. These grains are able to support α-amylase synthesis only in the presence of gibberellin A3 (GA3). In the absence of GA3 some protein synthesis occurs in embryoless grains during the
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20. Haloalkaliphilic maltotriose-forming alpha-amylase from the archaebacterium Natronococcus sp. strain Ah-36.
A haloalkaliphilic archaebacterium, Natronococcus sp. strain Ah-36, produced extracellularly a maltotriose-forming amylase. The amylase was purified to homogeneity by ethanol precipitation, hydroxylapatite chromatography, hydrophobic chromatography, and gel filtration. The molecular weight of the enzyme was estimated to be 74,000 by sodium dodecyl sulfate-po
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21. Visualizing Enzyme Secretion from Individual Barley (Hordeum vulgare) Aleurone Protoplasts.
A method was developed to detect [alpha]-amylase gene expression and [alpha]-amylase secretion from individual barley (Hordeum vulgare L. cv Himalaya) aleurone protoplasts. Protoplasts are incubated in liquid media with or without hormones and embedded in a thin film of agarose and starch, where they remain viable for up to 24 h. [alpha]-Amylase secreted by
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22. Gibberellin-Mediated Proteasome-Dependent Degradation of the Barley DELLA Protein SLN1 Repressor
DELLA proteins are nuclear repressors of plant gibberellin (GA) responses. Here, we investigate the properties of SLN1, a DELLA protein from barley that is destabilized by GA treatment. Using specific inhibitors of proteasome function, we show that proteasome-mediated protein degradation is necessary for GA-mediated destabilization of SLN1. We also show that
American Society of Plant Biologists.
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23. Wheat tetrameric inhibitors of insect α-amylases: Alloploid heterosis at the molecular level
Tetrameric inhibitors of heterologous α-amylases have been characterized in allohexaploid wheat, Triticum aestivum (genomes AABBDD), as well as in Triticum turgidum (AABB) and Triticum tauschii (DD). Their subunits have been identified as the previously described CM proteins. Single oligomeric species were observed in T. Turgidum (subunits CM2, CM3A, and CM
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24. Sugar-Induced Increase of Calcium-Dependent Protein Kinases Associated with the Plasma Membrane in Leaf Tissues of Tobacco.
The sugar-inducible expression of genes for sporamin and [beta]-amylase in leaf explants of sweet potato (Ipomoea batatas) and that of a [beta]-glucuronidase-fusion gene, with the promoter of the gene for [beta]-amylase in leaves of tobacco (Nicotiana tabacum), requires Ca2+ signaling (M. Ohto, K. Hayashi, M. Isobe, K. Nakamura [1995] Plant J 7: 297-307), an