6 Embryonic Stem Cells
Mostrando 1-12 de 134 artigos, teses e dissertações.
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1. Human Embryonic Stem Cell-Derived Mesenchymal Progenitor (hESCs-MP) Growth on Nanostructured Ti6Al4V Surfaces
Nanotexturing processes that focus on enhancing the bone-implant contact, such as electropolishing, have been proposed. The aim of this work was to evaluate the influence of Ti6Al4V surface morphology on human embryonic stem cell-derived mesenchymal progenitor (hESCs-MP) growth. Three surface treatments were used in this study: mechanically polished samples
Mat. Res.. Publicado em: 30/07/2018
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2. Combination of heterologous fibrin sealant and bioengineered human embryonic stem cells to improve regeneration following autogenous sciatic nerve grafting repair
Abstract Background Peripheral nerve injury is a worldwide clinical problem, and the preferred surgical method for treating it is the end-to-end neurorrhaphy. When it is not possible due to a large nerve gap, autologous nerve grafting is used. However, these surgical techniques result in nerve regeneration at highly variable degrees. It is thus very importa
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 24/05/2018
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3. Multipotent male germline stem cells (mGSCs) from neonate porcine testis
ABSTRACT Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis, during which unlimited spermatozoa is produced daily derived from SSCs in the testis throughout life of the male. Germline stem (GS) cells can be isolated from spermatogonia, which shared the characteristics of SSCs and embryonic stem cells (ESCs), and can be passaged stably in
Braz. arch. biol. technol.. Publicado em: 08/03/2016
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4. Isolation and characterization of mesenchymal progenitors derived from the bone marrow of goats native from northeastern Brazil
PURPOSE:To characterize bone marrow progenitors cells grown in vitro,using native goats from northeastern Brazil as animal model.METHODS:Ten northeastern Brazil native goats of both genders were used from the Piauí Federal University Agricultural Science Center's (UFPI) - Goat Farming Sector. Bone marrow aspirates where taken from the tibial ridge and seede
Acta Cir. Bras.. Publicado em: 2014-08
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5. Análise da função dos microRNAs na regulação da expressão de DNMT3B/Dnmt3b e MECP2/Mecp2 / Analysis of microRNAs function in the regulation of DNMT3B/Dnmt3b and MECP2/Mecp2 gene expression
A metilação do DNA em mamíferos é uma importante modificação epigenética, sendo essencial no silenciamento de DNAs repetitivos, de regiões que sofrem imprinting genômico e no estabelecimento do cromossomo X inativo em fêmeas. Existem 5 tipos de DNA Metiltransferases, tendo a DNMT3B um importante papel na metilação de novo. A MeCP2, por sua vez, �
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/01/2012
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6. Comparative study of ribosomal genes activity in leukocytes and gingival tissues from Down syndrome individuals, in relation to age. / Estudo comparativo da atividade dos genes ribossômicos em mucosa bucal (gengiva) e leucócitos de indivíduos com Síndrome de Down em relação à idade.
Cytogenetic and molecular studies have shown significant reduction in the ribosomal genes activity in lymphocyte and fibroblasts cells from older Trisomy 21 or Down syndrome (DS) individuals as well as in lymphocyte and neural cortical cells from Alzheimers disease patients. We have proposed the study of rRNA levels in older and younger DS subjects in two di
Publicado em: 2009
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7. Uso de matriz extracelular (Matrigel®) para estabelecimento de cultivo de células-tronco embrionárias de suínos e caracterização da expressão de moléculas associadas à pluripotência / Use of extracellular matrix (Matrigel®) for establishment of porcine embryonic stem cells and expression characterization of plurpotency related molecules
Establishment of embryonic stem cell (ESC) culture in pigs has not been achieved. Verification of pluripotency markers and differentiation in the three embryonic layers are necessary for validation of a pluripotent cell line. The objective of this study was to establish and characterize porcine ESC culture using Matrigel and compare the expression of pluripo
Publicado em: 2008
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8. In vitro differentiation of embryonic stem cells into lymphocyte precursors able to generate T and B lymphocytes in vivo.
Embryonic stem cells can be induced in vitro, by coculture with the stromal line RP.0.10 and a mixture of interleukins 3, 6, and 7, to differentiate into T (Joro75+) and B (B-220+) lymphocyte progenitors and other (Thy-1+, PgP-1+, c-kit+, Joro75-, B-220-, F4/80-, Mac-1-) hemopoietic precursors. The progeny of in vitro-induced embryonic stem cells can reconst
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9. Investigation of coelectroporation as a method for introducing small mutations into embryonic stem cells.
We have investigated coelectroporation as a method for introducing minor genetic changes into specific genes in embryonic stem cells. A selectable marker (neo) and a targeting replacement vector designed to insert a 4-bp insertion into exon 3 of the mouse hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene were coelectroporated into embryonic stem cel
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10. Targeted mutation of the Hprt gene in mouse embryonic stem cells.
The hypoxanthine-guanine phosphoribosyltransferase (Hprt) gene has been mutated in mouse blastocyst-derived embryonic stem cells by site-directed homologous recombination. Embryonic stem cells were electroporated in the presence of a targeting DNA fragment containing two specific features: (i) The targeting DNA contained a promoterless neomycin phosphotransf
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11. Enhanced hematopoietic differentiation of embryonic stem cells conditionally expressing Stat5
The signal transducer Stat5 plays a key role in the regulation of hematopoietic differentiation and hematopoietic stem cell function. To evaluate the effects of Stat5 signaling in the earliest hematopoietic progenitors, we have generated an embryonic stem cell line in which Stat5 signaling can be induced with doxycycline. Ectopic Stat5 activation at the poin
National Academy of Sciences.
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12. Germ-line transmission of a planned alteration made in a hypoxanthine phosphoribosyltransferase gene by homologous recombination in embryonic stem cells.
Embryonic stem cells (derived from 129/Ola mice) containing a mutant hypoxanthine phosphoribosyltransferase gene that had been corrected in vitro in a planned manner by homologous recombination were injected into blastocysts obtained from C57BL/6J mice. The injected blastocysts were introduced into pseudopregnant female mice to complete their development. El