5 Nc Hcv Region
Mostrando 1-12 de 12 artigos, teses e dissertações.
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1. Investigação epidemiológica e molecular da infecção pelo vírus da hepatite C em usuários de drogas ilícitas no Brasil Central / Epidemiologic and molecular investigation of hepatitis C virus infection among illicit drug users in Central Brazil
Hepatitis C virus (HCV) infection is an important problem of public health. Drug users (DU) constitute a group of frequent exposure to HCV and little is known about this infection in DU in Brazil. This study aimed to investigate the seroepidemiological and molecular profile of HCV infection among drug users in Central Brazil. A total of 691 DU, being 102 inj
Publicado em: 2009
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2. "Sequenciamento da região NS5A do genoma do vírus da hepatite C, genótipo 3, de pacientes brasileiros com infecção crônica" / Sequencing of NS5A region of HCV genotype 3 in brazilian patienwith chronic disease
The aim of this study was to analyse the sequences of fragments of E2 and NS5A regions from 33 outpatients infected with HCV genotype 3, including cirrhotic (C) and non-cirrhotic (NC) patients that have responded (R) or not (NR) to treatment. In the E2 region, we did observe few amino acids changes between patients without statistical significance. In the NL
Publicado em: 2006
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3. "Hepatite C: transmissão entre casais" / Hepatitis C: transmission between couples.
RESUMO Cavalheiro, NP. Hepatite C: transmissão entre casais. São Paulo, 2004. 111p. Tese (Doutorado) - Faculdade de Medicina, Universidade de São Paulo. Introdução: A ocorrência e eficiência da transmissão sexual do VHC na ausência de outros fatores de risco ainda é muito controversa. Foram investigados e analisados 24 casais, ambos cônjuges infec
Publicado em: 2004
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4. Sequence analysis of the 5' noncoding region of hepatitis C virus.
We have determined the nucleotide sequence of the 5' noncoding (NC) region of the hepatitis C virus (HCV) genome in 44 isolates from around the world. We have identified several HCV isolates with significantly greater sequence heterogeneity than reported previously within the 5' NC region. The most distantly related isolates were only 90.1% identical. Nucleo
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5. Clinical Evaluation of Two Methods for Genotyping Hepatitis C Virus Based on Analysis of the 5′ Noncoding Region
We compared the performance characteristics of a standardized direct sequencing method (TRUGENE HCV 5′NC; Visible Genetics Inc., Toronto, Ontario, Canada) and a reverse hybridization line probe assay (INNO-LiPA HCV II; Bayer Corp., Tarrytown, N.Y.) for genotyping of hepatitis C virus (HCV). Both methods are based on detection of sequence heterogeneity in t
American Society for Microbiology.
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6. Importance of primer selection for the detection of hepatitis C virus RNA with the polymerase chain reaction assay.
We compared four primer sets from conserved regions of the hepatitis C virus (HCV) genome for their ability to detect HCV RNA in a "nested" cDNA polymerase chain reaction assay on sera from 114 anti-HCV antibody-positive individuals from around the world. The different primer sets had equivalent sensitivity, detecting less than 1 chimpanzee ID50 (dose that i
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7. Genotyping of Hepatitis C Virus Isolates using CLIP Sequencing
Determination of hepatitis C virus (HCV) genotypes and subtypes has become increasingly important for the clinical management and prognosis of HCV infections. The aim of the present study was to assess the specificity and reliability of a newly developed, commercially available HCV genotyping kit (TRUGENE HCV 5′NC genotyping kit). This technique utilizes P
American Society for Microbiology.
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8. Comparison of Hepatitis C Virus NS5b and 5′ Noncoding Gene Sequencing Methods in a Multicenter Study
A national evaluation study was performed in 11 specialized laboratories with the objective of assessing their capacities to genotype hepatitis C virus (HCV) and define the applicability of a given genotyping method. The panel consisted of 14 samples positive for HCV RNA of different genotypes (including 3 samples with two different artificially mixed genoty
American Society for Microbiology.
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9. At least 12 genotypes of hepatitis C virus predicted by sequence analysis of the putative E1 gene of isolates collected worldwide.
In a previous study we sequenced the 5' noncoding (NC) region of 44 isolates of hepatitis C virus (HCV) and identified heterogeneous domains that provided evidence for additional genetic groups of HCV not previously recognized. In this study we have determined the complete nucleotide sequence of the putative envelope 1 (E1) gene in 51 HCV isolates from aroun
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10. Nested Restriction Site-Specific PCR To Detect and Type Hepatitis C Virus (HCV): a Rapid Method To Distinguish HCV Subtype 1b from Other Genotypes
Genotypic differentiation of hepatitis C virus (HCV) has become an integral part of clinical management and epidemiologic studies of hepatitis C infections. Thus, it is extremely important in areas such as the Czech Republic, where current instrumentation and kits for assessing HCV infection are too costly for widespread use. We describe a new and relatively
American Society for Microbiology.
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11. Hepatitis C Virus Genotyping Based on 5′ Noncoding Sequence Analysis (Trugene)
Hepatitis C virus (HCV) genotyping of samples from 184 patients with chronic HCV infection by the Trugene 5′NC genotyping kit, based on sequence analysis of the 5′ noncoding region (5′ NCR), and the InnoLiPA assay was evaluated. In addition to these methods, the 184 samples were also analyzed by sequencing of part of the NS5B of the HCV genome after in
American Society for Microbiology.
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12. Hepatitis C Virus (HCV) Genotypes in the Caribbean Island of Martinique: Evidence for a Large Radiation of HCV-2 and for a Recent Introduction from Europe of HCV-4
Molecular epidemiological studies of hepatitis C virus (HCV) in the Caribbean may help to specify the origin and spread of HCV infection. Indeed, the Caribbean population is intermixed from European and African origins and geographically close to the American continent. We characterized HCV genotypes in the Caribbean island of Martinique. HCV genotypes were
American Society for Microbiology.