2 Deuteration
Mostrando 13-24 de 26 artigos, teses e dissertações.
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13. Preparation of partially 2H/13C-labelled RNA for NMR studies. Stereo-specific deuteration of the H5′′ in nucleotides
An effective in vitro enzymatic synthesis is described for the production of nucleoside triphosphates (NTPs) which are stereo-specifically deuterated on the H5′′ position with high selectivity (>98%), and which can have a variety of different labels (13C, 15N, 2H) in other positions. The NTPs can subsequently be employed in the enzymatic synthesis of RNA
Oxford University Press.
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14. Structural studies of the primary donor cation radical P870+· in reaction centers of Rhodospirillum rubrum by electron-nuclear double resonance in solution
The light-induced cation radical of the primary electron donor, P870+·, in photosynthetic reaction centers from Rhodospirillum rubrum G-9, has been investigated by electron-nuclear double resonance (ENDOR) in liquid aqueous solution. The measured hyperfine coupling constants are assigned to specific molecular positions by partial deuteration. Comparison wit
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15. The NMR structure of the 47-kDa dimeric enzyme 3,4-dihydroxy-2-butanone-4-phosphate synthase and ligand binding studies reveal the location of the active site
Recent developments in NMR have extended the size range of proteins amenable to structural and functional characterization to include many larger proteins involved in important cellular processes. By applying a combination of residue-specific isotope labeling and protein deuteration strategies tailored to yield specific information, we were able to determine
The National Academy of Sciences.
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16. Asymmetry in the 50S Ribosomal Subunit of Escherichia coli
A substantial separation of the centers of mass of the protein and RNA portions of the 50S ribosomal subunit has been achieved using neutron scattering. This separation reveals that the subunit has a protein-rich side, a finding which is inconsistent with many models proposed for the structure. By analyzing the variation of the radius of gyration of the subu
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17. H-bonded N-heterocyclic base-pair phototautomerizational potential barrier and mechanism: The 7-azaindole dimer
A theoretical analysis of the double proton transfer (PT) in a hydrogen-bonded N-heterocyclic base pair is presented. The calculated (time-dependent density functional theory) double PT barrier calculated for the concerted process of the 7-azaindole C2h dimer in the first excited singlet electronic state S1 conforms well to the kinetic data and the photophys
National Academy of Sciences.
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18. Localizing the Membrane Binding Region of Group VIA Ca2+-independent Phospholipase A2 Using Peptide Amide Hydrogen/Deuterium Exchange Mass Spectrometry*
The Group VIA-2 Ca2+-independent phospholipase A2 (GVIA-2 iPLA2) is composed of seven consecutive N-terminal ankyrin repeats, a linker region, and a C-terminal phospholipase catalytic domain. No structural information exists for this enzyme, and no information is known about the membrane binding surface. We carried out deuterium exchange experiments with the
American Society for Biochemistry and Molecular Biology.
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19. Modulation of neocarzinostatin-mediated DNA double strand damage by activating thiol: deuterium isotope effects.
The neocarzinostatin chromophore causes double-strand damage at AGC sequences on DNA by concomitant 1'-oxidation at C and 5'-oxidation at the T on the complementary strand. The extent of this damage is dependent upon the structure of the thiol used for activation. Deuterium isotope effects suggest that this dependence on thiol structure may be due to interna
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20. TROSY in triple-resonance experiments: New perspectives for sequential NMR assignment of large proteins
The NMR assignment of 13C, 15N-labeled proteins with the use of triple resonance experiments is limited to molecular weights below ∼25,000 Daltons, mainly because of low sensitivity due to rapid transverse nuclear spin relaxation during the evolution and recording periods. For experiments that exclusively correlate the amide proton (1HN), the amide nitroge
The National Academy of Sciences.
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21. Interaction of transition metal ions with Z form poly d(A-C).poly d(G-T) and poly d(A-T) studied by I.R. spectroscopy.
Interactions between Ni2+, Co2+ and purine bases have been studied by I.R. spectroscopy in the case of double stranded regularly alternating purine-pyrimidine polynucleotides poly d(A-T), poly d(A-C).poly d(G-T) and poly d(G-C). The spectra of polynucleotide films have been recorded in hydration and salt content conditions which correspond to the obtention o
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22. Comparative structural analysis of cytidine, ethenocytidine and their protonated salts. II. IR spectral studies.
The IR spectra of crystalline cytidine (Cyd), ethenocytidine (epsilon Cyd), and their hydrochlorides (Cyd-Hcl and epsilon CyD-HCl) have been analyzed to determine the spectroscopic manifestations of the structural differences that were previously established for these nucleosides from X-ray studies. O,N-Deuteration of the samples turned out to be a successfu
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23. Comparison of poly(dG-dC).poly(dG-dC) conformations in oriented films and in solution.
The structures of B and Z forms of poly(dG-dC).poly(dG-dC) in oriented hydrated films have been investigated by infrared spectroscopy. First, the infrared linear dichroism spectrum of poly(dG-dC).poly(dG-dC) in B form (high relative humidity and 2 M Na+) was recorded. The spectrum of the Z form was recorded at a lower relative humidity and higher sodium cont
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24. Resonance Raman studies of bathorhodopsin: Evidence for a protonated Schiff base linkage
A dual beam pump/probe technique has been used with a 585-nm probe wavelength to obtain maximal resonance enhancement of the Raman lines of bathorhodopsin in a photostationary steady-state mixture at -160°C. These studies show that bathorhodopsin has a protonated Schiff base vibration at 1657 cm-1 which shifts upon deuteration to 1625 cm-1. Within our exper