1 Dnmt3b
Mostrando 1-12 de 40 artigos, teses e dissertações.
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1. Stability of XIST repression in relation to genomic imprinting following global genome demethylation in a human cell line
DNA methylation is essential in X chromosome inactivation and genomic imprinting, maintaining repression of XIST in the active X chromosome and monoallelic repression of imprinted genes. Disruption of the DNA methyltransferase genes DNMT1 and DNMT3B in the HCT116 cell line (DKO cells) leads to global DNA hypomethylation and biallelic expression of the imprin
Braz J Med Biol Res. Publicado em: 17/10/2014
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2. Avaliação dos Efeitos Antineoplásicos da Zebularina em Linhagens Pediátricas de Leucemia Linfoide Aguda. / Evaluation of Antineoplastic Effects of Zebularine on Childhood Acute Lymphoblastic Leukemia Cell Lines.
A leucemia linfóide aguda (LLA) é a neoplasia hematológica mais comum na infância e representa uma doença heterogênea em relação à biologia e ao prognóstico e seu tratamento consiste principalmente em quimioterapia. Apesar dos avanços no tratamento, cerca de 20% dos pacientes apresentam recaída da doença e/ou óbito indicando a necessidade de te
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/03/2012
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3. Análise da função dos microRNAs na regulação da expressão de DNMT3B/Dnmt3b e MECP2/Mecp2 / Analysis of microRNAs function in the regulation of DNMT3B/Dnmt3b and MECP2/Mecp2 gene expression
A metilação do DNA em mamíferos é uma importante modificação epigenética, sendo essencial no silenciamento de DNAs repetitivos, de regiões que sofrem imprinting genômico e no estabelecimento do cromossomo X inativo em fêmeas. Existem 5 tipos de DNA Metiltransferases, tendo a DNMT3B um importante papel na metilação de novo. A MeCP2, por sua vez, �
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/01/2012
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4. Multi-target siRNA based on DNMT3A/B homologous conserved region influences cell cycle and apoptosis of human prostate cancer cell line TSU-PR1
Abnormal genome hypermethylation participates in the tumorigenesis and development of prostate cancer. Prostate cancer cells highly express DNA methyltransferase 3 (DMNT3) family genes, essential for maintaining genome methylation. In the present study, multi-target siRNA, based on the homologous region of the DNMT3 family, was designed for the in vitro inve
Genetics and Molecular Biology. Publicado em: 16/02/2012
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5. Associação entre polimorfismos em genes envolvidos no metabolismo do folato e risco materno para a síndrome de Down.
A síndrome de Down (SD) é a cromossomopatia humana mais frequente e, na maioria dos casos (cerca de 90%), é caracterizada pela trissomia livre do cromossomo 21, resultante de falhas na segregação cromossômica durante a meiose materna. Estudos sugerem que a ocorrência da SD independente da idade materna está relacionada à hipometilação do DNA centr
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 05/08/2011
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6. Expressão imunoistoquímica das enzimas DNA metiltransferases 1, 3A e 3B em leucoplasias e carcinomas de células escamosas bucais
Objetivos: Neste estudo foi investigada a expressão das enzimas DNA metiltransferases (DNMTs) 1, 3a e 3b em carcinomas escamocelulares (CEC) e leucoplasias bucais, relacionando-a com a graduação histopatológica e presença de displasia epitelial, respectivamente, bem como com os parâmetros clínicos dos pacientes. Metodologia: A técnica de imunoistoqu�
Publicado em: 2009
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7. Análise funcional dos genes Xist e DNMT1 na manutenção do processo de inativação do cromossomo X humano através do silenciamento gênico por RNAi / Functional analysis of XIST and DNMT1 genes in the maintenance of X chromosome inactivation process in human through gene silencing by RNAi
X chromosome inactivation (XCI) is the phenomenon through which one of the X chromosomes in female mammals is silenced to achieve dosage compensation related to males. It involves the expression of XIST gene exclusively from the inactive X, and the association of its RNA in cis in this chromosome. This leads to a series of epigenetic modifications in the chr
Publicado em: 2008
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8. Mecanismos associados à perda de expressão do gene de galectina-3 em um modelo de progressão de melanoma murino / Mechanisms associated to the loss of galectin-3 gene expression in a model of murine melanoma progression
Galectin-3 is a b-galactoside-binding animal lectin, shown to be involved in tumor progression and metastasis. Galectin-3 expression has been found altered along tumor progression of different tumors. In some types of cancers such as thyroid carcinoma and bladder carcinoma, galectin-3 expression has been found increased, whereas in tumors such as breast carc
Publicado em: 2007
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9. Enzymatic properties of de novo-type mouse DNA (cytosine-5) methyltransferases
We have purified GST-fused recombinant mouse Dnmt3a and three isoforms of mouse Dnmt3b to near homogeneity. Dnmt3b3, an isoform of Dnmt3b, did not have DNA methylation activity. Dnmt3a, Dnmt3b1 or Dnmt3b2 showed similar activity toward poly(dG-dC)-poly(dG-dC) for measuring de novo methylation activity, and toward poly(dI-dC)-poly(dI-dC) for measuring total a
Oxford University Press.
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10. Establishment and Maintenance of Genomic Methylation Patterns in Mouse Embryonic Stem Cells by Dnmt3a and Dnmt3b
We have previously shown that the DNA methyltransferases Dnmt3a and Dnmt3b carry out de novo methylation of the mouse genome during early postimplantation development and of maternally imprinted genes in the oocyte. In the present study, we demonstrate that Dnmt3a and Dnmt3b are also essential for the stable inheritance, or “maintenance,” of DNA methylat
American Society for Microbiology.
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11. Preference of DNA Methyltransferases for CpG Islands in Mouse Embryonic Stem Cells
Many CpG islands have tissue-dependent and differentially methylated regions (T-DMRs) in normal cells and tissues. To elucidate how DNA methyltransferases (Dnmts) participate in methylation of the genomic components, we investigated the genome-wide DNA methylation pattern of the T-DMRs with Dnmt1-, Dnmt3a-, and/or Dnmt3b-deficient ES cells by restriction lan
Cold Spring Harbor Laboratory Press.
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12. Cooperativity between DNA Methyltransferases in the Maintenance Methylation of Repetitive Elements
We used mouse embryonic stem (ES) cells with systematic gene knockouts for DNA methyltransferases to delineate the roles of DNA methyltransferase 1 (Dnmt1) and Dnmt3a and -3b in maintaining methylation patterns in the mouse genome. Dnmt1 alone was able to maintain methylation of most CpG-poor regions analyzed. In contrast, both Dnmt1 and Dnmt3a and/or Dnmt3b
American Society for Microbiology.