Fusarium Sporotrichioides
Mostrando 1-12 de 45 artigos, teses e dissertações.
-
1. Toxina T-2 e alterações do crescimento endocondral em frangos de corte
Foi testada a habilidade da toxina T-2, produzida por Fusarium sporotrichioides Sherb e veiculada por milho experimentalmente contaminado, em induzir alterações da placa epifisária proximal do tibiotarso de frangos de corte. Pintos de um dia, todos machos e da linhagem Hubbard, foram alimentados com ração básica a base de milho e soja, na qual todo o m
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Publicado em: 2001-06
-
2. Histomorfometria e função da tireóide de frangos de corte após ingestão por curto período de toxina T-2 de Fusarium sporotrichioides
Determinaram-se a histomorfometria e a função da tireóide de frangos de corte após ingestão de toxina T-2 de Fusarium sporotrichioides, veiculada na ração por curto período. Foram utilizados 30 pintos da linhagem Hubbard, todos machos e com um dia de idade, distribuídos ao acaso em dois grupos. O grupo tratado recebeu ração contaminada com 2,64 mg
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Publicado em: 2001-02
-
3. Apoptose na depleção linfocitária induzida pela toxina T-2 em frangos de corte. Histomorfometria da bolsa de Fabricius
Estudou-se, pela histomorfometria da bolsa de Fabrício, o envolvimento da apoptose como mecanismo de depleção linfocitária em frangos de corte, após ingestão de toxina T-2 de Fusarium sporotrichioides veiculada pela ração. Foram utilizados 42 pintos de um dia de idade, distribuídos ao acaso em três grupos. O grupo tratado (n = 15) recebeu diariamen
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Publicado em: 2000-12
-
4. Formation of moniliformin by Fusarium sporotrichioides and Fusarium culmorum.
Two strains of Fusarium sporotrichioides and one strain of F. culmorum were shown to produce the mycotoxin moniliformin in rice culture. Identification was by reverse-phase liquid chromatography, thin-layer chromatography, and mass spectrometry.
-
5. Regulation of Trichodiene Synthase in Fusarium sporotrichioides and Gibberella pulicaris (Fusarium sambucinum)
The regulation of trichodiene synthase (TS) and its relationship to trichothecene biosynthesis was investigated in Fusarium sporotrichioides NRRL 3299 and Gibberella pulicaris R-6380. Cultures were analyzed for the presence of TS activity, trichothecenes, and immunodetectable TS polypeptide over a time period of 144 h. Enzyme activity increased from barely d
-
6. Disruption of TRI101, the Gene Encoding Trichothecene 3-O-Acetyltransferase, from Fusarium sporotrichioides
We screened a Fusarium sporotrichioides NRRL 3299 cDNA expression library in a toxin-sensitive Saccharomyces cerevisiae strain lacking a functional PDR5 gene. Fourteen yeast transformants were identified as resistant to the trichothecene 4,15-diacetoxyscirpenol, and each carried a cDNA encoding the trichothecene 3-O-acetyltransferase that is the F. sporotric
American Society for Microbiology.
-
7. Ancymidol blocks trichothecene biosynthesis and leads to accumulation of trichodiene in Fusarium sporotrichioides and Gibberella pulicaris.
Ancymidol, a plant growth regulator, inhibited biosynthesis of diacetoxyscirpenol by Gibberella pulicaris (Fusarium sambucinum) in a defined liquid medium. Ancymidol also inhibited biosynthesis of T-2 toxin by a wild-type strain of Fusarium sporotrichioides and biosynthesis of diacetoxyscirpenol, deacetylated calonectrin, and dideacetylated calonectrin by mu
-
8. Mycotoxin production by Fusarium oxysporum and Fusarium sporotrichioides isolated from Baccharis spp. from Brazil.
Fusarium oxysporum isolated from roots of and soil around Baccharis species from Brazil produced the trichothecenes T-2 toxin, HT-2 toxin, diacetoxyscirpenol, and 3'-OH T-2 (TC-1), whereas Fusarium sporotrichioides from the same source produced T-2 toxin, HT-2 toxin, acetyl T-2, neosolaniol, TC-1, 3'-OH HT-2 (TC-3), iso-T-2, T-2 triol, T-2 tetraol, and the n
-
9. Detection of T-2 toxin in Fusarium sporotrichioides-infected corn by enzyme-linked immunosorbent assay.
A competitive enzyme-linked immunosorbent assay was used to screen for T-2 toxin in Fusarium sporotrichioides -infected corn. The assay detected T-2 toxin in diluted methanol extracts of corn samples at concentrations of 0.05 ng/ml. In infected corn samples, enzyme-linked immunosorbent assay and gas-liquid chromatography estimations of T-2 toxin concentratio
-
10. Trichothecenes accumulated in liquid culture of a mutant of Fusarium sporotrichioides NRRL 3299.
A UV-generated mutant of Fusarium sporotrichioides NRRL 3299 was altered in its ability to biosynthesize T-2 toxin, as shown by a rapid screen with monoclonal antibodies to T-2. This stable mutant accumulated two trichothecenes that were not observed in liquid cultures of the parent strain. The two compounds were identified as 3,15-diol 12,13-epoxytrichothec
-
11. Isolation and characterization of two new trichothecenes from Fusarium sporotrichioides strain M-1-1.
Two new trichothecenes were isolated along with T-2 toxin, neosolaniol, and HT-2 toxin from the culture filtrate of Fusarium sporotrichioides strain M-1-1. The structures of the new toxins were characterized to be 4 beta, 8 alpha-diacetoxy-12,13-epoxytrichothec-9-ene-3 alpha, 15-diol (designated NT-1) and 4 beta-acetoxy-12,13-epoxy-trichothec-9-ene-3 alpha,8
-
12. New modified trichothecenes accumulated in solid culture by mutant strains of Fusarium sporotrichioides.
Mutant strains of Fusarium sporotrichioides NRRL 3299 deficient in the ability to synthesize T-2 toxin were examined on solid rice medium. Five novel alicyclic trichothecenes were isolated: 11 alpha-hydroxytrichodiene; tricho-9-ene-2 alpha,3 alpha,11 alpha-triol; tricho-9-ene-2 alpha,3 alpha,8 alpha,11 alpha-tetraol; tricho-9-ene-2 alpha,3 alpha,8 beta,11 al