Epsilon Toxin
Mostrando 1-12 de 25 artigos, teses e dissertações.
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1. Mapping of the continuous epitopes displayed on the Clostridium perfringens type D epsilon-toxin
Abstract The epsilon toxin, produced by Clostridium perfringens, is responsible for enterotoxemia in ruminants and is a potential bioterrorism agent. In the present study, 15 regions of the toxin were recognized by antibodies present in the serum, with different immunodominance scales, and may be antigen determinants that can be used to formulate subunit vac
Braz. J. Microbiol.. Publicado em: 2017-07
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2. Naturally acquired antibodies against Clostridium perfringens epsilon toxin in goats.
2008
Veterinary Immunology Immunopathology. Publicado em: 2011
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3. Isolamento e tipifícação genotípica de Clostridium Perfringens em frangos de corte.
Clostridium perfringens is an anaerobic Gram-positive bacterium which causes gaseous gangrene and enterotoxaemias in humans and domestic animals besides being the primary cause of necrotic enteritis in poultry. Clostridium perfringens strains were isolated 171/250 (68.4%) from the intestinal content of broiler chickens sampled in a slaughterhouse in Pará de
Publicado em: 2007
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4. Enterotoxemia em bovino
This report describes a case of bovine enterotoxaemia in Morro da Garça, Minas Gerais, Brazil. Clostridium perfringens type D was isolated in pure culture and was characterized by biochemical reactions and PCR. By the mouse neutralization test, the presence of epsilon toxin from intestinal content was detected.
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Publicado em: 2006-10
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5. Produção e caracterização de anticorpos monoclonais anti épsilon toxina de Clostridiun perfrigens tipo D
Monoclonal antibodies reactive against épsilon toxin from Clostridium perfringens type D were produced. Myeloma cell lines Sp2/0. Ag14 and P3-X63-Ag8.653 were fused with spleen cells from mice BALB/c immunized with epsilon toxoid. After fusion 59 wells were positive for the Sp2/0. Ag14 cell line and 19 for the P3-X63-Ag8.653, representing 6.68 and 6.59% res
Publicado em: 2006
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6. Detection of alpha, beta and epsilon toxin genes of Clostridium perfringens isolated from cattle?s clinical samples by polimerase chain reaction / Detecção dos genes das toxinas alfa, beta e épsilon de Clostridium perfringens isolados a partir de amostras clínicas de bovinos pela reação em cadeia da polimerase
Clostridium perfringens is an anaerobic micro-organism that is present in the soil and gastrointestinal tract of mammals. It causes food poisoning in humans, enterotoxemic diseases in domestic animals and gas gangrene in both. C. perfringens is classified into five types (A, B, C, D and E) according to the production of four major toxins (alpha, beta, epsilo
Publicado em: 2004
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7. Clostridium perfringens epsilon-toxin acts on MDCK cells by forming a large membrane complex.
Epsilon-toxin is produced by Clostridium perfringens types B and D and is responsible for a rapidly fatal enterotoxemia in animals, which is characterized by edema in several organs due to an increase in blood vessel permeability. The Madin-Darby canine kidney (MDCK) cell line has been found to be susceptible to epsilon-toxin (D. W. Payne, E. D. Williamson,
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8. Tryptophan content of Clostridium perfringens epsilon toxin.
The tryptophan content of Clostridium perfringens epsilon toxin was investigated. When the tryptophan content was determined by amino acid analysis after the hydrolysis of epsilon prototoxin with methanesulfonic acid containing 3-(2-aminoethyl)indole and by the spectrophotometric method with N-bromosuccinimide, the number of tryptophan residues was calculate
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9. Effect of Clostridium perfringens epsilon toxin on the cardiovascular system of rats.
Pressor activity was demonstrated by the intravenous injection of purified epsilon toxin of Clostridium perfringens type D but not by that of epsilon prototoxin. The activity was completely abolished by C. perfringens type B or D antiserum, but not by type A, C, or E antiserum. The rise in blood pressure caused by the toxin was accompanied by a decrease in t
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10. High-affinity binding of Clostridium perfringens epsilon-toxin to rat brain.
125I-epsilon-toxin showed high affinity to rat brain homogenates and synaptosomal membrane fractions, having single binding phases with dissociation constants (Kds) of 2.5 and 3.3 nM, respectively. Treatment of synaptosomal membrane fractions with pronase and neuraminidase lowered the binding of the labeled toxin, whereas treatment with trypsin and phospholi
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11. Accumulation of Clostridium perfringens Epsilon-Toxin in the Mouse Kidney and Its Possible Biological Significance
In this paper we show that Clostridium perfringens epsilon-toxin accumulates predominantly in the mouse kidney, where it is distributed mainly in glomeruli, capillaries, and collecting ducts. Although some pycnotic and exfoliated epithelial cells were observed in distal tubuli and collecting ducts, there were no findings indicative of severe renal injury. Bi
American Society for Microbiology.
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12. Cloning and nucleotide sequencing of the Clostridium perfringens epsilon-toxin gene and its expression in Escherichia coli.
The sequence of 20 amino acids from the N terminus of Clostridium perfringens epsilon-toxin was determined. Some differences between this sequence and the previously published sequence (A. S. Bhown and A. F. S. A. Habeeb, Biochem. Biophys. Res. Commun. 78:889-896, 1977) were found. A degenerate 23-bp pair oligonucleotide probe was designed from the amino aci