Dre Pcr
Mostrando 1-9 de 9 artigos, teses e dissertações.
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1. Os métodos de extração de DNA e a qualidade DA Taq polimerase podem melhorar a tipagem molecular de M. tuberculosis por DRE-PCR
Amplificação de duplo elemento repetido (DRE) por PCR é um método simples para tipagem de Mycobacterium tuberculosis, entretanto falha ou a baixa resolução das bandas na amplificação compromete a eficiência do método. Com o objetivo de verificar se estes problemas podem ou não ser minimizados pela utilização de diferentes procedimentos de extra�
Brazilian Journal of Microbiology. Publicado em: 2007-09
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2. Epidemiological characterization of resistance and PCR typing of shigella flexneri and shigella sonnei strains isolated from bacillary dysentery cases in Southeast Brazil / Caracterização biologica e molecular de amostras de shigella flexneri e shigella sonnei isoladas da regiao de Campinas-SP
Shigella spp are gram-negative, anaerobic facultative, non-motile, and non-sporulated bacilli of the Enterobacteriaceae family, responsible for Shigellosis or the Bacillary Dysentery (BD) disease, an important cause of worldwide morbidity and mortality. The pathogenic determinants of Shigella spp include high molecular weight plasmids responsible for the bac
Publicado em: 2007
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3. Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains
Simple double repetitive element polymerase chain reaction (MaDRE-PCR) and Pvu II-IS1245 restriction fragment length polymorphism (RFLP) typing methods were used to type 41 Mycobacterium avium isolates obtained from 14 Aids inpatients and 10 environment and animals specimens identified among 53 mycobacteria isolated from 237 food, chicken, and pig. All envir
Memórias do Instituto Oswaldo Cruz. Publicado em: 2005-11
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4. Drug resistance and genotypes of strains of Mycobacterium tuberculosis isolated from human immunodeficiency virus-infected and non-infected tuberculosis patients in Bauru, São Paulo, Brazil
Little is known about transmission and drug resistance of tuberculosis (TB) in Bauru, State of São Paulo. The objective of this study was to evaluate risk factors for transmission of Mycobacterium tuberculosis strains in this area. Strains were collected from patients attended at ambulatory services in the region and susceptibility towards the main first li
Memórias do Instituto Oswaldo Cruz. Publicado em: 2002-12
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5. Molecular Fingerprinting of Mycobacterium tuberculosis Isolates Obtained in Havana, Cuba, by IS6110 Restriction Fragment Length Polymorphism Analysis and by the Double-Repetitive-Element PCR Method
Mycobacterium tuberculosis sputum isolates from 38 patients, obtained in the first 6 months of 1997 in Havana, Cuba, were characterized by IS6110 restriction fragment length polymorphism (RFLP) analysis and the double-repetitive-element PCR (DRE-PCR) method. Among 41 strains from 38 patients, 24 and 25 unique patterns, and 5 and 4 cluster patterns, were foun
American Society for Microbiology.
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6. Spoligotyping Followed by Double-Repetitive-Element PCR as Rapid Alternative to IS6110 Fingerprinting for Epidemiological Studies of Tuberculosis
A total of 129 clinical isolates of Mycobacterium tuberculosis representing 91 patients were typed by a combination of direct-repeat (DR)-based spoligotyping and an inter-IS6110–PGRS (polymorphic GC-rich region)–PCR, also designated double-repetitive-element PCR (DRE-PCR). During the first phase of this investigation, 72 clinical strains representing 52
American Society for Microbiology.
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7. Molecular Typing of Mycobacterium tuberculosis Based on Variable Number of Tandem DNA Repeats Used Alone and in Association with Spoligotyping
Fingerprinting based on variable numbers of tandem DNA repeats (VNTR), a recently described methodology, was evaluated for molecular typing of Mycobacterium tuberculosis in an insular setting. In this study, VNTR fingerprinting was used alone or as a second-line test in association with spoligotyping, double-repetitive-element PCR (DRE-PCR), and IS6110 restr
American Society for Microbiology.
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8. Comparison of Methods Based on Different Molecular Epidemiological Markers for Typing of Mycobacterium tuberculosis Complex Strains: Interlaboratory Study of Discriminatory Power and Reproducibility
In this study, the currently known typing methods for Mycobacterium tuberculosis isolates were evaluated with regard to reproducibility, discrimination, and specificity. Therefore, 90 M. tuberculosis complex strains, originating from 38 countries, were tested in five restriction fragment length polymorphism (RFLP) typing methods and in seven PCR-based assays
American Society for Microbiology.
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9. The Pepper Transcription Factor CaPF1 Confers Pathogen and Freezing Tolerance in Arabidopsis1
An ERF/AP2-type transcription factor (CaPF1) was isolated by differential-display reverse transcription-PCR, following inoculation of the soybean pustule pathogen Xanthomonas axonopodis pv glycines 8ra, which induces hypersensitive response in pepper (Capsicum annuum) leaves. CaPF1 mRNA was induced under conditions of biotic and abiotic stress. Higher levels
American Society of Plant Biologists.