Cysteine Endopeptidase
Mostrando 1-12 de 24 artigos, teses e dissertações.
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1. Localization and function of Rhipicephalus (Boophilus) microplus vitellin-degrading cysteine endopeptidase
The tick Rhipicephalus (Boophilus) microplus is an important parasite of cattle in many areas of the tropics. Characterization of molecules involved in mechanisms such as vitellogenesis and embryo development may contribute to a better understanding of this parasite’s physiology. The vitellin-degrading cysteine endopeptidase (VTDCE) is the most active enzy
Publicado em: 2011
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2. A Boophilus microplus vitellin-degrading cysteine endopeptidase
Here we describe the purification and characterization of a vitellin (VT) degrading cysteine endopeptidase (VTDCE) from eggs of the hard tick Boophilus microplus. A homogeneous enzyme preparation was obtained by chromatographic fractionation on ion-exchange and gel filtration columns and an autolysis step. This step consisted of incubation of a semipurified
Publicado em: 2011
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3. Endocrinologia e controle da vitelogênese em carrapatos = Endocrinology and control of tick vitellogenesis / Endocrinology and control of tick vitellogenesis
Background: Ticks are distributed worldwide, with impacts on human and animal health. The cattle tick Rhipicephalus (Boophilus) microplus is the main parasite that affects livestock in tropical and subtropical regions of the world, causing large economical losses. Tick control methods are based on the application of chemical acaricides, which has resulted in
Publicado em: 2011
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4. Endocrinologia e controle de vitelogênese em carrapatos / Endocrinology and control of tick vitellogenesis
Background: Ticks are distributed worldwide, with impacts on human and animal health. The cattle tick Rhipicephalus (Boophilus) microplus is the main parasite that affects livestock in tropical and subtropical regions of the world, causing large economical losses. Tick control methods are based on the application of chemical acaricides, which has resulted in
Publicado em: 2011
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5. Degradação de vitelina e hemoglobina no carrapato bovino Rhipicephalus (Boophilus) microplus
O presente trabalho caracterizou alguns aspectos enzimáticos da digestão de vitelina e de hemoglobina no carrapato bovino Rhipicephalus (Boophilus) microplus. Um novo protocolo foi desenvolvido para a purificação de uma cisteíno endopeptidase de larvas do carrapato (R. microplus Larval Cysteine Endopeptidase; RmLCE). A seqüência parcial de aminoácido
Publicado em: 2010
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6. A reprodução em carrapatos e a avaliação de uma enzima de destaque neste processo como antígeno vacinal contra Rhipicephalus (Boophilus) microplus
Os carrapatos estão distribuídos por todo mundo e impactam tanto a saúde animal quanto humana. A espécie Rhipicephalus (Boophilus) microplus é o principal parasita que afeta a bovinocultura nas regiões tropicais e subtropicais do globo, causando grandes prejuísos econômicos. Os métodos de controle estão baseados no uso de acaricidas químicos, o qu
Publicado em: 2008
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7. A cysteine endopeptidase isolated from castor bean endosperm microbodies processes the glyoxysomal malate dehydrogenase precursor protein.
A plant cysteine endopeptidase with a molecular mass of 35 kD was purified from microbodies of germinating castor bean (Ricinus communis) endosperm by virtue of its capacity to specifically process the glyoxysomal malate dehydrogenase precursor protein to the mature subunit in vitro. Processing of the glyoxysomal malate dehydrogenase precursor occurs sequent
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8. Structure of the streptococcal endopeptidase IdeS, a cysteine proteinase with strict specificity for IgG
Pathogenic bacteria have developed complex and diverse virulence mechanisms that weaken or disable the host immune defense system. IdeS (IgG-degrading enzyme of Streptococcus pyogenes) is a secreted cysteine endopeptidase from the human pathogen S. pyogenes with an extraordinarily high degree of substrate specificity, catalyzing a single proteolytic cleavage
National Academy of Sciences.
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9. C-Terminal KDEL Sequence of A KDEL-Tailed Cysteine Proteinase (Sulfhydryl-Endopeptidase) Is Involved in Formation of KDEL Vesicle and in Efficient Vacuolar Transport of Sulfhydryl-Endopeptidase1
Sulfhydryl-endopeptidase (SH-EP) is a papain-type vacuolar proteinase expressed in cotyledons of germinated Vigna mungo seeds, and the enzyme possesses a C-terminal propeptide containing KDEL tail, an endoplasmic reticulum retention signal for soluble proteins. SH-EP is transported to vacuoles via a KDEL vesicle (KV) through a Golgi complex-independent r
The American Society for Plant Biologists.
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10. Histidine and Aspartic Acid Residues Important for Immunoglobulin G Endopeptidase Activity of the Group A Streptococcus Opsonophagocytosis-Inhibiting Mac Protein
The secreted Mac protein made by serotype M1 group A Streptococcus (GAS) (designated Mac5005) inhibits opsonophagocytosis and killing of GAS by human polymorphonuclear neutrophils. This protein also has cysteine endopeptidase activity against human immunoglobulin G (IgG). Site-directed mutagenesis was used to identify histidine and aspartic acid residues imp
American Society for Microbiology.
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11. A peptide-hormone-inactivating endopeptidase in Xenopus laevis skin secretion.
An endopeptidase was isolated from Xenopus laevis skin secretions. This enzyme, which has an apparent molecular mass of 100 kDa, performs a selective cleavage at the Xaa-Phe, Xaa-Leu, or Xaa-Ile bond (Xaa = Ser, Phe, Tyr, His, or Gly) of a number of peptide hormones, including atrial natriuretic factor, substance P, angiotensin II, bradykinin, somatostatin,
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12. Identification of active-site residues of the adenovirus endopeptidase.
Multiple sequence alignment of the 12 adenovirus endopeptidases known to date identified a number of conserved residues which might be important for enzyme activity. Eleven mutants were created in the cloned gene by site-directed mutagenesis to identify the active site of this thiol endopeptidase. Analysis of the proteolytic activity in a crude system using