Buccal Cell Dna
Mostrando 1-12 de 12 artigos, teses e dissertações.
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1. Class III Malocclusion: Missense Mutations in DUSP6 Gene
Abstract Objective: To determine the DUSP6 gene mutation in three generations of Malaysian Malay subjects having Class III malocclusion. Material and Methods: Genetic analyses of DUSP6 gene were carried out in 30 subjects by selecting three individuals representing three generations, respectively, from ten Malaysian Malay families having Class III malocclu
Pesqui. Bras. Odontopediatria Clín. Integr.. Publicado em: 10/10/2019
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2. Buccal cells submitted to three different storage conditions before DNA extraction
This study evaluated quantitatively and qualitatively the effect of the storage time of samples before the application of the cell lysis solution (CLS) for extracting DNA from buccal cells (BC). BC from the upper and lower gutter region were collected from 5 volunteers using special cytobrushes (Gentra), totaling 3 collections for each individual. In the con
Journal of Applied Oral Science. Publicado em: 2009-04
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3. Comparison between DNA obtained from buccal cells of the upper and lower gutter area
O objetivo do presente estudo foi comparar quantitativamente e qualitativamente o DNA extraído de células epiteliais bucais coletadas do fundo de sulco superior e inferior. Foram coletadas células bucais do fundo de sulco superior (n=15) e inferior (n=15) de 15 voluntários utilizando escovas citológicas especiais (Gentra), totalizando 2 coletas por volu
Brazilian Dental Journal. Publicado em: 2009
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4. Analise do padrão de metilação da região promotora do gene humano PAX9 (-947 - +251) e analise in vitro da sua atividade transcricional / New insights into methylation pattern and transcriptional activity of human PAX9 gene (-947 - +251) in vitro
PAX9 is a key regulator during tooth development and plays an essential role in the patterning of murine and human dentition. In humans, mutations in PAX9 are associated with unique phenotypes of familial tooth agenesis that mainly involve posterior teeth. The objectives of this study were to gain new insights into the transcriptional activity and DNA methyl
Publicado em: 2009
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5. Lack of association between matrix metalloproteinase-1 (MMP-1) promoter polymorphism and risk of renal cell carcinoma
OBJECTIVE: Investigate the possible association of insertion/deletion (2G/G) polymorphism at nucleotide -1607 of the MMP-1 promoter with the development and progression of renal cancer MATERIALS AND METHODS: In this study, we genotyped 217 individuals, 99 patients with renal cell carcinoma (RCC) and 118 controls without cancer. DNA specimens were extracted f
International braz j urol. Publicado em: 2007-10
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6. Apoptose induzida por extrato aquoso de Pteridium aquilinum em células de glândula submandibular humana (HSG) e de epitélio bucal (OSCC-3)
The bracken Pteridium aquilinum is considered one of the most important toxic plants, not only for its extensive geographic distribution, but also for provoking, in different animal species that use it as food, severe poisoning and the development of tumors in the digestory and urinary tracts. Moreover, epidemiologic studies have related the high incidence o
Publicado em: 2006
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7. APOE genotyping and its association with cognitive deficits in children with diarrhea and malnutrition in the Northeast-Brazil / Genotipagem da apolipoproteína E e sua associação com déficits cognitivos em crianças com diarréia e desnutrição no Nordeste do Brasil
Polymorphisms in the apolipoprotein E (APOE) have constituted the major rationale to identify potential risk groups for developing late-onset Alzheimer s disease and help to predict recovery of cognitive function after brain injury. However, the APOE impact on cognitive development in children living in poor areas of the developing world, where we have disco
Publicado em: 2004
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8. Detailed chromosomal and molecular genetic analysis of single cells by whole genome amplification and comparative genomic hybridisation.
Molecular genetic analysis of isolated single cells and other minute DNA samples is limited because there is insufficient DNA to perform more than one independent PCR amplification. One solution to this problem is to first amplify the entire genome, thus providing enough DNA for numerous subsequent PCRs. In this study we have investigated four different meth
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9. Clonally expanded mtDNA point mutations are abundant in individual cells of human tissues
Using single-cell sequence analysis, we discovered that a high proportion of cells in tissues as diverse as buccal epithelium and heart muscle contain high proportions of clonal mutant mtDNA expanded from single initial mutant mtDNA molecules. We demonstrate that intracellular clonal expansion of somatic point mutations is a common event in normal human tiss
National Academy of Sciences.
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10. Functional Characterization of Low-Prevalence Missense Polymorphisms in the UDP-Glucuronosyltransferase 1A9 Gene
The UDP-glucuronosyltransferase (UGT) 1A9 has been shown to play an important role in the detoxification of several carcinogens and clearance of anticancer and pain medications. The goal of the present study was to identify novel polymorphisms in UGT1A9 and characterize their effect on glucuronidation activity. The UGT1A9 gene was analyzed by direct sequenci
The American Society for Pharmacology and Experimental Therapeutics.
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11. Sequential expression and cooperative interaction of c-Ha-ras and c-erbB genes in in vivo chemical carcinogenesis.
The level of expression of several cellular protooncogenes is examined at different stages of 7,12-dimethylbenzanthracene (DMBA)-induced tumor development in hamster buccal pouch epithelium (HBPE). Results presented demonstrate overexpression of c-Ha-ras gene at a very early stage of tumor development, and this elevated level of expression of the gene persis
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12. Expression, cloning, and characterization of a Candida albicans gene, ALA1, that confers adherence properties upon Saccharomyces cerevisiae for extracellular matrix proteins.
Adherence of Candida albicans to host tissues is a necessary step for maintenance of its commensal status and is likely a necessary step in the pathogenesis of candidiasis. The extracellular matrix (ECM) proteins are some of the host tissue and plasma proteins to which C. albicans adheres through adhesins located on the fungal cell surface. To isolate genes