X-ray Analysis Studies of Elements Stored in Protein Body Globoid Crystals of Triticum Grains 1

AUTOR(ES)

Lott, John N. A.

RESUMO

Energy-dispersive x-ray analysis was used to investigate the elemental storage within protein bodies, specifically the globoid crystals, in grains of wheat. Areas of the grain investigated included various parts of the embryo, the aleurone layer plus starchy endosperm near the embryo and the aleurone layer plus starchy endosperm farthest from the embryo. Variations did occur grain-to-grain, cell-to-cell and, in certain regions, intracellularly. No protein bodies with electron-dense globoid crystals were found in the starchy endosperm. Generally globoid crystals contained P, K, and Mg in all areas investigated. Globoid crystals from the aleurone layer farthest from the embryo on occasion contained Ca, whereas aleurone globoid crystals near the embryo sometimes contained Fe. In most of the embryo regions examined, a few globoid crystals contained Ca along with P, K, and Mg. No specific pattern to the Ca distribution could be found. Welldefined elemental distribution occurred with Mn. Manganese was found only in globoid crystals located in the base and midregions of the stele in the radicle. Thus, in wheat there is some specific distribution of minerals dependent upon cell type and/or position in the grain.

Documentos Relacionados

• X-RAY ANALYSIS OF PREFERRED ORIENTATION OF QUARTZ CRYSTALS IN THREE LINEATED QUARTZITES*
• Protein Body Composition in Cuburbita maxima Cotyledons as Determined by Energy Dispersive X-Ray Analysis 1
• X-ray diffraction studies of fibers and crystals of deoxygenated sickle cell hemoglobin.
• X-ray Laue diffraction from crystals of xylose isomerase.
• Iron distances in hemoglobin: comparison of x-ray crystallographic and extended x-ray absorption fine structure studies.