USO DE DILUENTES ALTERNATIVOS A BAIXAS TEMPERATURAS NA MANUTENÇÃO DA QUALIDADE ESPERMÁTICA DO SÊMEN SUÍNO / USE OF ALTERNATIVE SOLVENTS AT LOW TEMPERATURES IN THE MAINTENANCE OF SPERM QUALITY OF SWINE SEMEN

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

29/06/2012

RESUMO

Countless studies have been conducted with the purpose of improving the conservation of boar semen, either with the use of diluents or alternative temperature ranges, or adding antioxidants or amino acid to the diluent. The present study aimed to define an alternative diluent and temperature preservation of boar semen and evaluate the effects of adding trolox or betaine in the maintenance of sperm quality. The semen of eight boars were collected weekly through the gloved hand technique and underwent laboratory analysis of vigor and motility on the day of collection and during the storage period (D0 to D4), evaluated for vitality, acrosome integrity and test hyposmotic on D0 and D4. Initially, the ejaculates were preserved in three diluents: BTS, water coconut powder (ACP) and skimmed milk powder (LPD) and subjected to two storage temperatures 17 and 10 C, a total of six treatments. The best defined of these treatment was added three different concentrations of trolox (1000, 2000 and 3000 μM/ 100 mL) and betaine (1, 2 and 3g / 100 mL). Of the diluents tested, the LPD 10 C provided better preservation of the spermatic motility and vigor, and higher rate of vitality and a higher percentage of cells with intact acrosome. Given these results, the LPD 10 C presented as the best diluent to perform the following steps. The samples added with different concentrations of trolox were no better when compared to control treatment in any of the analyzes. High concentrations of trolox (2000 and 3000 μM) had negative effects on the vigor and sperm motility. The samples added with betaine, were not observed beneficial effects. Betaine likely due to its osmotic effects, reduced vigor and motility of the samples, this effect was greater when it increased the concentration of this amino acid. The LPD can be used as a diluent for boar semen conditioned at 10 C to be used for up to 24 hours after dilution. You cannot prove the beneficial effects of trolox in the preservation of boar semen in medium based on LPD. And betaine had detrimental effects on sperm quality when added to the diluent based on the LPD, at concentrations above 1% by storage temperature to 10 C.

ASSUNTO(S)

coconut powdered water skimmed powdered milk antioxidant sêmen suíno água de coco em pó leite em pó desnatado antioxidantes reproducao animal boar semen

ACESSO AO ARTIGO

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