Use of sodC versus ctrA for real-time polymerase chain reaction-based detection of Neisseria meningitidis in sterile body fluids

AUTOR(ES)

Higa, Fábio Takenori, Fukasawa, Lucila Okuyama, Gonçalves, Maria Gisele, Salgado, Maristela Marques, Lemos, Ana Paula Silva de, Harrison, Lee H, Oliveira, Priscilla Lima de, Silva, Carla Naufal da, Sacchi, Claudio Tavares

FONTE

Mem. Inst. Oswaldo Cruz

DATA DE PUBLICAÇÃO

2013-04

RESUMO

We evaluated the use of a newly described sodC-based real-time-polymerase chain reaction (RT-PCR) assay for detecting Neisseria meningitidis in normally sterile sites, such as cerebrospinal fluid and serum. The sodC-based RT-PCR assay has an advantage over ctrA for detecting nongroupable N. meningitidis isolates, which are commonly present in asymptomatic pharyngeal carriage. However, in our study, sodC-based RT-PCR was 7.5% less sensitive than ctrA. Given the public health impact of possible false-negative results due to the use of the sodC target gene alone, sodC-based RT-PCR for the diagnosis of meningococcal meningitis should be used with caution.

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