Toxicology analysis of antidepressants in whole blood with gas chromatography and nitrogen-phosphorus detection / Análise toxicológica de antidepressivos em sangue total por cromatografia em fase gasosa com detector de nitrogênio e fósforo

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

A gas chromatographic method was developed to determine antidepressants most prescribed in Brazil and their metabolites (amitriptyline, imipramine, clomipramine, desmethylclomipramine, desipramine, nortriptyline, fluoxetine, norfluoxetine, and sertralina) in whole blood, using solid phase extraction and gas chromatography with nitrogen-phosphorus detection. The solid-phase extraction (SPE) with abselutTM NEXUS was applied in an innovative manner. The extraction procedure consists of the dilution of 0.5mL of whole blood in buffer (pH 9.5), application of the sample in the cartridge, washing with buffer (pH 9.5) and elution of the analytes with dichloromethane/isopropanol (17/3, v/v). The limit of detection (LOD) and quantification (LLOQ) were from 0.1mg/L to 0.4mg/L and from 0.4mg/L to 1.6mg/L, respectively. Etidocaine was used as internal standard. The method was precise, specific and linear in the studied concentration (range from LLOQ to 12mg/L). The average recovery of all analytes was 65,5%. Forensic and clinical emergency samples were submitted to the validated method.

ASSUNTO(S)

antidepressants whole blood cromatografia gasosa extração em fase sólida solid phase extraction intoxication intoxicação sangue total antidepressivos gas chromatography

ACESSO AO ARTIGO

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