The chemical study of biological activities from the mansoa hirsuta Bignoniaceae / Estudo químico e de atividades biológicas da mansoa hirsuta D.C (Bignoniaceae)

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

Plants of the Bignoniaceae family in Brazil, as Mansoa hirsuta, have medicinal use and some biological activities were already proved experimentally. This study aimed to isolate and identify compounds with activity anti-Leshmania amazonensis, anti-Tripanossoma cruzi, molluscicidal, larvicidal and immunomodulatory in plant extracts of Mansoa hirsuta D.C.. The botanist Teonildes Nunes collected and identified the plant material of the herbarium of the State University of Feira de Santana (HUEFS). The crude extract of the plant was obtained by extraction with ethanol (90%), while a liquid-liquid partition and a reverse phase extraction gave the subfractions. Then, the crude extract and subfractions were used in a phytochemical screening to identify the secondary metabolites. To determine the non-toxic of the extracts, mouse cells (spleen cells) were used. After the establishment of the optimum concentration, the anti-parasitic test took place. The parasites were cultivated in presence of the extract and subfractions and the inhibition percentile was taken by the MTT metabolism. The lymphoproliferation inhibition tests were carried out with the optimum concentrations by cultivating the cells in the presence of the extract and subfractions, as well concanovaline, a mitogenic compound. The proliferation was established by incorporating tritiated thymidine. The nitrite dosage was used to evaluate the nitric oxide (NO) inhibition when mouse peritoneal macrophages were cultivated in the presence of IFN-y, LPS and the extracts in the optimum concentration through the Griss s Method. The molluscicidal and larvicidal activities were performed in UFAL with concentrations previously found. The recovered ethanol from the crude extract partitioning, from Klavenger extraction with hexane and dichloromethane and the collected oil in the process of isolation from the acetate fraction were analysed in GC-MS. The oil was submitted to hydrolyze and a separation in a silica gel chromatographic column. The ethyl acetate and chlorophormic fractions were also partitioned, filtered and eluted in chromatographic columns to isolate compounds. The structural elucidation of the isolated substances was carried out using H and CNMR, IR and mass spectrometry. The isolated substances were sylilated with BSTFA and GC-MS analyzed to prove their purity. The screening showed the extracts contained several secondary metabolites: phenols, tannin, steroids and flavonoids. The bioassays revealed significant activities of some extract fractions above 60%. An immunosuppressive activity of 99.2% to lymphocytes was verified for the ethyl acetate extract (PFR), while a nitric oxide inhibition of 57.92% was found to this same extract. A tripanomicidal in the ethyl acetate and chloroform extracts (PLL) of 65.02% and 66.37% were found,respectively. The GC analyses showed the presence of diallyl di- and trisulphide in the recovered ethanol. In the Klavenger hexane fraction was identified a homologous series of n-alkanes and alkenes, while a series of alcohols and isobutyl butyrate ester in the dichloromethane extracts. The oil showed a homologous series of ethyl esters and some free fatty acids. The ursolic acid was isolated from the ethyl acetate fractions, while lupeol and a mixture of ß-sitosterol and stigmasterol were obtained in the chloroform fraction.

ASSUNTO(S)

atividades biológicas mansoa hirsuta mansoa hirsuta análise química chemical analysis quimica organica biological activities

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