Stability-indicating RP-HPLC method for simultaneous determination of gatifloxacin and flurbiprofen in binary combination
AUTOR(ES)
Khan, Islam Ullah, Razzaq, Syed Naeem, Mariam, Irfana, Ashfaq, Muhammad, Razzaq, Syed Saleem
FONTE
Quím. Nova
DATA DE PUBLICAÇÃO
2014-04
RESUMO
A stability-indicating RP-HPLC method is presented for determination of gatifloxacin and flurbiprofen in binary combination. Gatifloxacin, flurbiprofen and their degradation products were detected at 254 nm using a BDS Hypersil C8 (250 X 4.6 mm, 5 µm) column and mixture of 20 mM phosphate buffer (pH 3.0) and methanol 30:70 v/v as mobile phase. Response was linear over the range of 15-105 mg mL-1 for gatifloxacin (r² > 0.998) and of 1.5-10.5 mg mL-1 for flurbiprofen (r² > 0.999). The developed method efficiently separated the analytical peaks from degradation products (peak purity index > 0.9999). The method developed can be applied successfully for determination of gatifloxacin and flurbiprofen in human serum, urine, pharmaceutical formulations, and their stability studies.
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