Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil

AUTOR(ES)

Chávez, Juliana Helena, Reis, Vinicius Pinho dos, Silva, Jaqueline Raymondi, Laure, Helen Julie, Rosa, José Cesar, Fonseca, Benedito Antônio Lopes da, Figueiredo, Luiz Tadeu Moraes

FONTE

Rev. Soc. Bras. Med. Trop.

DATA DE PUBLICAÇÃO

2013-01

RESUMO

INTRODUCTION: West Nile virus (WNV) is a flavivirus with a natural cycle involving mosquitoes and birds. Over the last 11 years, WNV has spread throughout the Americas with the imminent risk of its introduction in Brazil. METHODS: Envelope protein domain III of WNV (rDIII) was bacterially expressed and purified. An enzyme-linked immunosorbent assay with WNV rDIII antigen was standardized against mouse immune fluids (MIAFs) of different flavivirus. RESULTS: WNV rDIII reacted strongly with St. Louis encephalitis virus (SLEV) MIAF but not with other flaviviruses. CONCLUSIONS: This antigen may be a potentially useful tool for serologic diagnosis and may contribute in future epidemiological surveillance of WNV infections in Brazil.

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