Produção e eficácia de anticorpos gerados contra glicolipídios de Paracoccidioides brasiliensis / Production and efficacy of antibodies generated against glycolipids of P. brasiliensis

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

10/10/2012

RESUMO

Paracoccidioidomycosis (PCM) is a systemic fungal infection most prevalent in Latin America, whose etiologic agent, Paracoccidioides brasiliensis, is a yeast with a complex cell wall structure consisting of proteins, glycoproteins, polysaccharides, lipids, and melanin polypeptides that gather physical, chemical and biological conditions to serve as antigens. Antibodies against antigens present in the wall of P. brasiliensis can interfere with the course of the disease. The mechanisms by which antibodies mediate protection against fungi are in the process of elucidation by several research groups. However, these molecules may be protective, providing increased efficiency of effector cells of the host against the fungus and modulating the production of cytokines, among other things. Treatment of PCM is relatively efficient, however, it takes long time and some patients drop out before it ends or do not follow the treatment properly resulting in reactivation of infectious focus. Accordingly, we evaluated the effect of passive transfer of antibodies against acidic glycolipids by prophylactic and therapeutic protocols in animals infected with P. brasiliensis. The results of in vivo tests, showed that the treated animals had a significant reduction in fungal load and tissue damage in the lungs. The noted increase in cytokine IFN-? and IL-12 modulated the immune response to Th1. In vitro assays have shown that treatment with polyclonal antibodies generated against the acidic glycolipids were able to increase the phagocytic activity, production of nitric oxide and killing of yeast Pb 18 by peritoneal macrophages. Treatment with antibodies against glycolipids antigens proved to be an important tool to search for the cure of PCM.

ASSUNTO(S)

antibodies anticorpos camundongos glicolipídeos glycolipids imunização passiva mice paracoccidioides paracoccidioides paracoccidioidomicose paracoccidioidomycosis passive immunization

ACESSO AO ARTIGO

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