Preparation and characterization of silk fibroin membranes to use as biomaterial / Obtenção e caracterização de membranas de fibroina de seda para aplicação como biomaterial

AUTOR(ES)

Grinia Michelle Nogueira

DATA DE PUBLICAÇÃO

2005

RESUMO

Fibroin is a fibrous protein, also found in silk. Recently, several researchers have investigated silk fibroin as a promising resource for biotechnology and biomedical materiaIs due to its mechanical resistance, biocompatibility and minimal inflammatory reaction. Based on these characteristics, other applications have been proposed, as wound protection, substrate for cell culture, enzyme immobilization and drug-release agents. In the literature, there are several preparation methods of dense silk fibroin membranes, but the preparation and characterization of the porous ones are less studied. In this work, dense and I porous silk fibroin membranes were prepared and characterized for application as biomaterial. Porous membranes were prepared from silk foam, a product of denaturation of protein from solution. The chemical and physical analysis of dense and porous membranes showed there are difference on their secondary structure, morphology and mechanical properties. The ethanol treatment promotes change on the secondary structure of dense and porous membranes. Porous membranes change their secondary structure from -"beta" sheet to random coil ("alfa"-form) conformation improving their elasticity and tensile resistance at break. The ethanol treatment on dense membranes promotes changes in the secondary structure from random coil to "beta" sheet conformation, and make them insoluble in water. The final membranes showed high elasticity and traction and punch resistance. From calorimetric analysis, we could observe that the degradation of fibroin starts at 300°C, for dense and porous membranes. Porous membranes have a potential as biomaterial or matrix for cell culture, as demonstrated from the preliminar in vitro cell culture tests. Morphology analysis also indicated that membrane pore size ranged from c.a. 200 to 400/um

ASSUNTO(S)

fibroin membranes fibroina biomateriais biomaterials seda membranas (tecnologia) silk

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