Phosphorylation of specific, distinct proteins in synaptosomes and axons from squid nervous system
AUTOR(ES)
Pant, Harish C.
RESUMO
Synaptosomes and axons from squid were incubated with [γ-32P]ATP or [32P]orthophosphate and specific, distinct proteins were found to be labeled in each preparation. In axoplasm, only the major 200,000 Mr neurofilament protein and a specific protein of ≈400,000 Mr were labeled, as reported previously [Pant, H. C., Shecket, G., Gainer, H. & Lasek, R. J. (1978) J. Cell Biol. 78, R23-R27]. These results were independent of whether the cosubstrates were 32PO42- or [γ-32P]ATP. However, synaptosomes lacked the 200,000 Mr neurofilament protein and several lower molecular weight proteins were labeled instead, the most prominent being a 47,000 Mr species. [γ-32P]ATP was much more effective in labeling the 47,000 Mr species than 32PO42-. Synaptosomes also contained a distinct 250,000 Mr protein species which, however, was not labeled.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=411804Documentos Relacionados
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