Unconventional vegetables collected in Brazil: chromosome number and description of nuclear DNA content
AUTOR(ES)
Silva, Luis Felipe Lima e, Techio, Vânia Helena, Resende, Luciane Vilela, Braz, Guilherme Tomaz, Resende, Kátia Ferreira Marques de, Samartini, Carolina Queiroz
FONTE
Crop Breed. Appl. Biotechnol.
DATA DE PUBLICAÇÃO
2017-12
RESUMO
Abstract The aim of this study was to perform chromosome counts and nuclear DNA quantification of the unconventional vegetables species: bertalha (Basella alba L.), vinagreira verde (Hibiscus sabdariffa L.), azedinha (Rumex acetosa L.), peixinho (Stachys byzantina K. Koch), and capuchinha (Tropaeolum majus L.). Metaphase chromosomes were obtained from the pre-treatment of root meristem with 8-hydroxyquinoline or colchicine and lides were prepared by the flame-drying technique and stained with 5% Giemsa. DNA quantification was performed by flow cytometry. Chromosome number and DNA content (pg) estimated for each species were: Rumex acetosa: 2n=2x=14 and 7.04 pg; Basella alba: 2n=2x=44 and 7.05 pg; Tropaeolum majus: 2n=2x=28 and 2.08 pg; Stachys byzantine: 2n=2x=30 and 1.54 pg, and Hibiscus sabdariffa: 2n=4x=72 and 5.12 pg.
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